Calcium store depletion in dimethyl BAPTA-loaded human platelets increases protein tyrosine phosphorylation in the absence of a rise in cytosolic calcium
| Autor: | Paul Sargeant, Stewart O. Sage, Richard W. Farndale |
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| Rok vydání: | 1994 |
| Předmět: |
Blood Platelets
Thapsigargin Fura-2 chemistry.chemical_element Protein tyrosine phosphatase Calcium chemistry.chemical_compound Cytosol Humans Dimethyl Sulfoxide Phosphorylation Tyrosine Egtazic Acid Chelating Agents Terpenes Tyrosine phosphorylation General Medicine Cell biology chemistry Biochemistry Intracellular |
| Zdroj: | Experimental Physiology. 79:269-272 |
| ISSN: | 0958-0670 |
| DOI: | 10.1113/expphysiol.1994.sp003762 |
| Popis: | The endomembrane Ca(2+)-ATPase inhibitor, thapsigargin, was used to deplete the intracellular Ca2+ stores of fura-2-loaded human platelets. In control cells, thapsigargin evoked a rise in cytosolic [Ca2+] and a substantial increase in protein tyrosine phosphorylation. Thapsigargin also evoked an increase in tyrosine phosphorylation in cells co-loaded with fura-2 and the Ca2+ chelator dimethyl BAPTA, such that the rise in cytosolic [Ca2+] was abolished. These data support the existence of a tyrosine phosphatase regulated by the Ca2+ content of the intracellular store, a requirement of the putative model for reciprocal control of Ca2+ entry by cytosolic and store [Ca2+] via protein tyrosine phosphorylation. |
| Databáze: | OpenAIRE |
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