Study on RNAi-based herbicide for Mikania micrantha
| Autor: | Yu Yu, Jingying Lin, Jiantao Mai, Chen Weizhao, Ling Rongsong, Beixin Mo, Liao Lingling, Guo Xiaolong |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Spray-induced gene silencing
GMOs genetically modified organisms QH301-705.5 HIGS host-induced gene silencing SEM scanning electron microscope Invasive plant Biomedical Engineering LHCs light-harvesting complexes Nucleic acid bioherbicide SIGS Spray-induced gene silencing Applied Microbiology and Biotechnology Article RNA interference Mikania micrantha Structural Biology Botany Genetics Biology (General) siRNA short interfering RNA qRT-PCR Quantitative real-time PCR biology biology.organism_classification RNP RNAi nanomicrosphere RNAi RNA interference shRNA short hairpin RNA dsRNA double-stranded RNA Chlorophyll a/b-binding protein TP248.13-248.65 Biotechnology |
| Zdroj: | Synthetic and Systems Biotechnology, Vol 6, Iss 4, Pp 437-445 (2021) Synthetic and Systems Biotechnology |
| ISSN: | 2405-805X |
| Popis: | The invasive plant Mikania micrantha Kunth (M. micrantha) from South America poses a significant threat to the stability and biodiversity of ecosystems. However, an effective and economical method to control M. micrantha is still lacking. RNA interference (RNAi) has been widely studied and applied in agriculture for trait improvement. Spray-induced gene silencing (SIGS) can produce RNAi silencing effects without introducing heritable modifications to the plant genome and is becoming a novel nontransformation strategy for plant protection. In this study, the genes encoding chlorophyll a/b-binding proteins were selected as targets of RNAi, based on high-throughput sequencing of M. micrantha transcriptome and bioinformatic analyses of sequence specificity. Three types of RNAi molecules, double-stranded RNA, RNAi nanomicrosphere, and short hairpin RNA (shRNA), with their corresponding short interfering RNA sequences were designed and synthesized for SIGS vector construction, from which each RNAi molecule was transcribed and extracted to be sprayed on M. micrantha leaves. Whereas water-treated control leaves remained green, leaves treated with RNAi molecules turned yellow and eventually wilted. Quantitative real-time PCR showed that the expression levels of target genes were significantly reduced in the RNAi-treated groups compared with those of the control, suggesting that all three types of RNAi herbicides effectively silenced the endogenous target genes, which are essential for the growth of M. micrantha. We also found that shRNA showed better silencing efficiency than the other two molecules. Taken together, our study successfully designed three types of RNAi-based herbicides that specifically silenced endogenous target genes and controlled the growth of M. micrantha. Moreover, we identified a gene family encoding chlorophyll a/b-binding proteins that is important for the growth and development of M. micrantha and could serve as potential targets for controlling the spread of M. micrantha. |
| Databáze: | OpenAIRE |
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