Characterization of mutations induced by ethylnitrosourea in seminiferous tubule germ cells of transgenic B6C3F1 mice

A.T transitions, all of which occurred at CpG dinucleotides. Fifteen of 22 sites (68%) found mutated 3 days after ENU treatment occurred at G.C base pairs, although some of these are expected to be spontaneous mutations. Ninety days after treatment, 13 of 19 sites (68%) found mutated occurred at A.T base pairs. The mutation spectra seen are consistent with proposed mechanisms of ENU mutagenesis and correlate with the in vivo spectra seen in ENU studies by using transmissibility assays and the hprt gene. These findings represent significant progress toward defining the in vivo spectra of ENU mutagenesis in mammalian germ cells. -->

ISSN:	1091-6490 0027-8424
DOI:	10.1073/pnas.91.14.6564
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fbf18b4547065c88e1fff69591da8af https://doi.org/10.1073/pnas.91.14.6564
Rights:	OPEN
Přírůstkové číslo:	edsair.doi.dedup.....8fbf18b4547065c88e1fff69591da8af
Autor:	G S Provost, J M Short
Rok vydání:	1994
Předmět:	Male Transgene Mutant Mice Inbred Strains Mice Transgenic Biology Mice medicine Animals Point Mutation Mutation frequency Gene Crosses Genetic Repetitive Sequences Nucleic Acid Sequence Deletion Base Composition Mice Inbred C3H Multidisciplinary Mutation Spectra Point mutation DNA Seminiferous Tubules Spermatozoa Molecular biology Mice Inbred C57BL Seminiferous tubule medicine.anatomical_structure Mutagenesis Ethylnitrosourea Mutation Female Research Article
Zdroj:	Proceedings of the National Academy of Sciences. 91:6564-6568
ISSN:	1091-6490 0027-8424
DOI:	10.1073/pnas.91.14.6564
Popis:	Transgenic B6C3F1 mice carrying a lacI target gene were exposed to acute and multiple doses of ethylnitrosourea (ENU), and germ cells from the seminiferous tubules were assayed for mutation 3 and 90 days after treatment. Relative to untreated controls, the mutation frequency increased 3.2- and 19.9-fold at 3 and 90 days after treatment, respectively. Mutant lacI genes recovered from untreated and treated groups were sequenced, and the spectra of mutations were determined. Eighty-five percent (11/13) of the spontaneous mutations resulted in G.C-->A.T transitions, all of which occurred at CpG dinucleotides. Fifteen of 22 sites (68%) found mutated 3 days after ENU treatment occurred at G.C base pairs, although some of these are expected to be spontaneous mutations. Ninety days after treatment, 13 of 19 sites (68%) found mutated occurred at A.T base pairs. The mutation spectra seen are consistent with proposed mechanisms of ENU mutagenesis and correlate with the in vivo spectra seen in ENU studies by using transmissibility assays and the hprt gene. These findings represent significant progress toward defining the in vivo spectra of ENU mutagenesis in mammalian germ cells.
Databáze:	OpenAIRE
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