Caffeine exerts a dual effect on capacitative calcium entry in Xenopus oocytes
| Autor: | Gérard Brûlé, Thibault Collin, Fabrice Matifat, Frédéric Hague |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
IBMX Xenopus Context (language use) chemistry.chemical_compound Caffeine Internal medicine medicine Animals Protein kinase A Receptor Microinjection Ion Transport biology Cell Biology biology.organism_classification Cyclic AMP-Dependent Protein Kinases Endocrinology chemistry Second messenger system Oocytes Biophysics Calcium Central Nervous System Stimulants Female Calcium Channels Signal Transduction |
| Zdroj: | Cellular Signalling. 12:31-35 |
| ISSN: | 0898-6568 |
| DOI: | 10.1016/s0898-6568(99)00074-1 |
| Popis: | Caffeine increases the amplitude of the Cl − currents evoked by capacitative Ca 2+ entry (CCE) on thapsigargin-treated Xenopus oocytes. The caffeine-induced potentiation of the CCE process appears to rest on two distinct and additive components. The first component involves the cAMP second messenger system since it can be mimicked by either IBMX perfusion or cAMP microinjection into the oocyte and inhibited by the PKA inhibitory peptide i-PKA. The second component, although activatory, is dynamically related to the caffeine-evoked inhibition of InsP 3 -mediated Ca 2+ release and may arise from an interaction between caffeine and the InsP 3 receptor in the context of a conformational coupling between the InsP 3 receptor and the channels responsible for CCE. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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