Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion
| Autor: | Melissa Faria, Demetrio Raldúa, Caterina Faggio, Natàlia Garcia-Reyero, Marina Bellot, Cristian Gómez-Canela, Jordi Mestres, Eva Prats, Maria Pagano, Josep M. Porta, Arnau Valls, Leobardo Manuel Gómez Oliván, Jonathan Ricardo Rosas Ramírez, Juliette Bedrossiantz |
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| Přispěvatelé: | Raldúa, Demetrio [0000-0001-5256-1641], Institut de Robòtica i Informàtica Industrial, Universitat Politècnica de Catalunya. KRD - Cinemàtica i Disseny de Robots, Raldúa, Demetrio |
| Rok vydání: | 2021 |
| Předmět: |
Insecticides
medicine.medical_specialty Environmental Engineering 010504 meteorology & atmospheric sciences Informàtica::Automàtica i control [Àrees temàtiques de la UPC] medicine.drug_class Escape response 010501 environmental sciences 01 natural sciences Fenitrothion chemistry.chemical_compound Automation Internal medicine Monoaminergic medicine Zebrafish larvae Neurotoxicity Animals Environmental Chemistry Waste Management and Disposal Endocrine disruptors Ecosystem Zebrafish 0105 earth and related environmental sciences Risk assessment Encocrine disruptors medicine.disease Pollution Acetylcholinesterase Acetylcholinesterase inhibitor Androgenic effects Predicted target profile Androgens Larva Androgen receptor Endocrinology chemistry Automation [Classificació INSPEC] |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) |
| Popis: | Fenitrothion is an organophosphorus insecticide usually found in aquatic ecosystems at concentrations in the range of low ng/L. In this manuscript we show that 24 h exposure to environmental concentrations of fenitrothion, from ng/L to low μg/L, altered basal locomotor activity, visual-motor response and acoustic/vibrational escape response of zebrafish larvae. Furthermore, fenitrothion and expression of gap43a, gfap, atp2b1a, and mbp exhibited a significant non-monotonic concentration-response relationship. Once determined that environmental concentrations of fenitrothion were neurotoxic for zebrafish larvae, a computational analysis identified potential protein targets of this compound. Some of the predictions, including interactions with acetylcholinesterase, monoamine-oxidases and androgen receptor (AR), were experimentally validated. Binding to AR was the most suitable candidate for molecular initiating event, as indicated by both the up-regulation of cyp19a1b and sult2st3 and the non-monotonic relationship found between fenitrothion and the observed responses. Finally, when the integrity of the monoaminergic system was evaluated, altered levels of L-DOPA, DOPAC, HVA and 5-HIAA were found, as well as a significant up-regulation of slc18a2 expression at the lowest concentrations of fenitrothion. These data strongly suggest that concentrations of fenitrothion commonly found in aquatic ecosystems present a significant environmental risk for fish communities. We thank Dr. Harold A. Burgess for providing Flote software package and advice with the kinematic analysis setup for the acoustic/vibrational evoked escape response. This work was supported by the Spanish Government with FEDER Funds (CTM2017-83242- R; D.R.) and the network of recognized research groups by the Catalan Government (2017 SGR_902). Melissa Faria acknowledges financial support from the Beatriu de Pinós programme (grant Nº: 2016 BP 00233) provided by the Secretariat of Universities and Research department of the Ministry for Business and Knowledge, Catalonia Government. Maria Pagano benefited from a PhD grant (Avviso 24/2018) given by Sicily region, supported by FSE 2014-2020. Mention of specific products or trade names does not indicate endorsement by the US federal government. |
| Databáze: | OpenAIRE |
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