Effects of Cancer Presence and Therapy on the Platelet Proteome

Autor: Henk J. Broxterman, Henk L. Dekker, Henk M.W. Verheul, Mirjam G.A. oude Egbrink, Connie R. Jimenez, Jaco C. Knol, Thang V. Pham, Maudy Walraven, Siamack Sabrkhany, Sander R. Piersma, Inge de Reus, Arjan W. Griffioen
Přispěvatelé: Internal medicine, AII - Cancer immunology, CCA - Cancer biology and immunology, Medical oncology laboratory, CCA - Imaging and biomarkers, CCA - Cancer Treatment and quality of life, Amsterdam Neuroscience - Neurodegeneration, Fysiologie, RS: Carim - B03 Cell biochemistry of thrombosis and haemostasis, Medical oncology
Jazyk: angličtina
Rok vydání: 2021
Předmět:
BIOMARKER
0301 basic medicine
Male
Proteome
Angiogenesis
platelets
biomarkers
cancer
antitumor therapy
proteomics
Pharmacology
Proteomics
Digestive System Neoplasms
ANGIOGENESIS
Tumours of the digestive tract Radboud Institute for Health Sciences [Radboudumc 14]
0302 clinical medicine
TUMOR
STAGE
Platelet
Biology (General)
S100A9
Spectroscopy
General Medicine
Middle Aged
CALPROTECTIN
Computer Science Applications
Chemistry
030220 oncology & carcinogenesis
Biomarker (medicine)
Female
Blood Platelets
QH301-705.5
Antineoplastic Agents
Catalysis
Article
MECHANISMS
S100A8
Inorganic Chemistry
03 medical and health sciences
medicine
Biomarkers
Tumor
Humans
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
LABEL-FREE
Aged
business.industry
Organic Chemistry
Cancer
medicine.disease
030104 developmental biology
CELL-DEATH
Cancer biomarkers
PARANEOPLASTIC THROMBOCYTOSIS
business
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, 22
International Journal of Molecular Sciences, 22(15):8236. Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences, Vol 22, Iss 8236, p 8236 (2021)
International Journal of Molecular Sciences; Volume 22; Issue 15; Pages: 8236
Walraven, M, Sabrkhany, S, Knol, J C, Dekker, H, de Reus, I, Piersma, S R, Pham, T V, Griffioen, A W, Broxterman, H J, Oude Egbrink, M, Verheul, H M W & Jimenez, C R 2021, ' Effects of Cancer Presence and Therapy on the Platelet Proteome ', International Journal of Molecular Sciences, vol. 22, no. 15, 8236 . https://doi.org/10.3390/ijms22158236
International journal of molecular sciences, 22(15):8236. Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences, 22, 15
ISSN: 1422-0067
1661-6596
DOI: 10.3390/ijms22158236
Popis: Contains fulltext : 238212.pdf (Publisher’s version ) (Open Access) Platelets are involved in tumor angiogenesis and cancer progression. Previous studies indicated that cancer could affect platelet content. In the current study, we investigated whether cancer-associated proteins can be discerned in the platelets of cancer patients, and whether antitumor treatment may affect the platelet proteome. Platelets were isolated from nine patients with different cancer types and ten healthy volunteers. From three patients, platelets were isolated before and after the start of antitumor treatment. Mass spectrometry-based proteomics of gel-fractionated platelet proteins were used to compare patients versus controls and before and after treatment initiation. A total of 4059 proteins were detected, of which 50 were significantly more abundant in patients, and 36 more in healthy volunteers. Eight of these proteins overlapped with our previous cancer platelet proteomics study. From these data, we selected potential biomarkers of cancer including six upregulated proteins (RNF213, CTSG, PGLYRP1, RPL8, S100A8, S100A9) and two downregulated proteins (GPX1, TNS1). Antitumor treatment resulted in increased levels of 432 proteins and decreased levels of 189 proteins. In conclusion, the platelet proteome may be affected in cancer patients and platelets are a potential source of cancer biomarkers. In addition, we found in a small group of patients that anticancer treatment significantly changes the platelet proteome.
Databáze: OpenAIRE
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