Determination of ribavirin in chicken muscle by quick, easy, cheap, effective, rugged and safe method and liquid chromatography-tandem mass spectrometry
| Autor: | Jian Zhao, Ruo-Xia Chen, Lie Zhu, Yong Zhu, Yan Lv, Yin-Liang Wu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Meat Coefficient of variation 030106 microbiology Clinical Biochemistry Quechers Mass spectrometry 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Ribavirin Animals Sample preparation Muscle Skeletal Reproducibility Chromatography 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine Repeatability Drug Residues 0104 chemical sciences chemistry Linear Models Chickens Ammonium acetate Chromatography Liquid |
| Zdroj: | Journal of Chromatography B. :55-60 |
| ISSN: | 1570-0232 |
| Popis: | A new analytical method for the determination of ribavirin in chicken muscle using a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method and liquid chromatography-tandem mass spectrometry (LC-MS-MS) was developed and validated. Samples were extracted with acidified methanol (methanol:acetic acid, 99:1, v/v). The extract was further purified by QuEChERS method using primary-secondary amine (PSA) and C18. Finally, the extract was dried by nitrogen under 45°C and reconstituted in water. The separation was performed on a Hypercarb analytical column under a gradient elution. The mobile phase was composed of water buffered with ammonium acetate (2.0mM) and acetonitrile. The proposed method was validated according to the European Commission Decision 2002/657/EC. The values of the decision limit (CCα) and the detection capability (CCβ) were 1.1 and 1.5μg/kg, respectively. The mean recoveries of ribavirin ranged from 94.2% to 99.2%. The repeatability (expressed as coefficient of variation, CVr) of the method ranged from 4.5% to 4.9% and the reproducibility (CVR) of the method ranged from 4.8% to 5.4%. The method is demonstrated to be suitable for the determination of ribavirin in chicken muscle in conformity with the current EU performance requirements through validation. The total time required for the analysis of one sample, including sample preparation, was about 45min. |
| Databáze: | OpenAIRE |
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