Sarcoplasmic reticulum Ca2+ decreases with age and correlates with the decline in muscle function in Drosophila
| Autor: | Maria Teresa Alonso, Alba Delrio-Lorenzo, Javier García-Sancho, Jonathan Rojo-Ruiz |
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| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Junta de Castilla y León |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Sarcopenia
medicine.medical_specialty SERCA Sarcoplasmic reticulum Biology Retículo sarcoplasmático Homeostasis del calcio 03 medical and health sciences 0302 clinical medicine Calcium imaging Internal medicine Calcium homeostasis medicine 030304 developmental biology Calcium metabolism 0303 health sciences Ryanodine receptor Endoplasmic reticulum Retículo endoplasmático Cell Biology medicine.disease Drosophila melanogaster Endocrinology medicine.symptom 030217 neurology & neurosurgery Homeostasis Muscle contraction |
| Zdroj: | UVaDOC. Repositorio Documental de la Universidad de Valladolid instname Digital.CSIC. Repositorio Institucional del CSIC |
| Popis: | Producción Científica Sarcopenia, the loss of muscle mass and strength associated with age, has been linked to impairment of the cytosolic Ca2+ peak that triggers muscle contraction, but mechanistic details remain unknown. Here we explore the hypothesis that a reduction in sarcoplasmic reticulum (SR) Ca2+ concentration ([Ca2+]SR) is at the origin of this loss of Ca2+ homeostasis. We engineered Drosophila melanogaster to express the Ca2+ indicator GAP3 targeted to muscle SR, and we developed a new method to calibrate the signal into [Ca2+]SR in vivo. [Ca2+]SR fell with age from ∼600 µM to 50 µM in close correlation with muscle function, which declined monotonically when [Ca2+]SR was Ministerio de Economía, Industria y Competitividad (grant BFU2017-83066-P) Junta de Castilla y León (project GR175) |
| Databáze: | OpenAIRE |
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