[68 ga]ga-nodaga-e[(Crgdyk)]2 angiogenesis pet/mr in a porcine model of chronic myocardial infarction
| Autor: | Cecilie Bjørstrup Larsen, Andreas Kjaer, Helle Hjorth Johannesen, Thomas Lund Andersen, Trine Pagh Ludvigsen, Lisbeth H. Olsen, Mathias Loft, Jacob E. Møller, Mette Flethøj, Jeppe Kirchhoff, Thomas Jespersen, Simon Bentsen, Andreas Clemmensen, Karina Poulsdóttir Debes |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Medicine (General) Positron emission tomography positron emission tomography Angiogenesis Clinical Biochemistry Ischemia Infarction large animal model Late gadolinium enhancement cardiac imaging 030218 nuclear medicine & medical imaging 03 medical and health sciences angiogenesis 0302 clinical medicine R5-920 Magnetic resonance imaging In vivo medicine magnetic resonance imaging Myocardial infarction cardiovascular diseases 030304 developmental biology 0303 health sciences medicine.diagnostic_test business.industry Interesting Images medicine.disease Large animal model medicine.anatomical_structure myocardial infarction PET/MRI late gadolinium enhancement cardiovascular system Immunohistochemistry business Cardiac imaging Artery |
| Zdroj: | Bentsen, S, Clemmensen, A, Loft, M, Flethøj, M, Debes, K P, Ludvigsen, T P, Larsen, C B, Kirchhoff, J, Olsen, L H, Møller, J E, Andersen, T L, Johannesen, H H, Jespersen, T & Kjaer, A 2021, ' [ 68 ga]ga-nodaga-e[(Crgdyk)] 2 angiogenesis pet/mr in a porcine model of chronic myocardial infarction ', Diagnostics, vol. 11, no. 10, 1807 . https://doi.org/10.3390/diagnostics11101807 Diagnostics, Vol 11, Iss 1807, p 1807 (2021) Bentsen, S, Clemmensen, A, Loft, M, Flethøj, M, Debes, K P, Ludvigsen, T P, Larsen, C B, Kirchhoff, J, Olsen, L H, Møller, J E, Andersen, T L, Johannesen, H H, Jespersen, T & Kjaer, A 2021, ' [ 68 Ga]Ga-NODAGA-E[(cRGDyK)] 2 Angiogenesis PET/MR in a Porcine Model of Chronic Myocardial Infarction ', Diagnostics, vol. 11, no. 10, 1807 . https://doi.org/10.3390/diagnostics11101807 Diagnostics |
| DOI: | 10.3390/diagnostics11101807 |
| Popis: | Angiogenesis is crucial in tissue repair and prevents scar tissue formation following an ischemic event such as myocardial infarction. The ischemia induces formation of new capillaries, which have high expression of integrin αv β3 . [68 Ga]Ga-NODAGA-E[(cRGDyK)]2 ([68 Ga]Ga-RGD) is a promising PET-radiotracer reflecting angiogenesis by binding to integrin αv β3 . A Göttingen mini-pig underwent transient catheter-induced left anterior descending artery (LAD) occlusion for 120 min, and after 8 weeks was imaged on a Siemens mMR 3T PET/MR system. A large antero-septal infarction was evident by late gadolinium enhancement (LGE) on the short axis and 2–4 chamber views. The infarcted area corresponded to the area with high [68 Ga]Ga-RGD uptake on the fused PET/MR images, with no uptake in the healthy myocardium. To support the hypothesis that [68 Ga]Ga-RGD uptake reflects angiogenesis, biopsies were sampled from the infarct border and healthy myocardium. Expression of αv β3 was evaluated using immunohistochemistry. The staining showed higher αv β3 expression in the capillaries of the infarct border compared to those in the healthy myocardium. These initial data confirm in vivo detection of angiogenesis using [68 Ga]Ga-RGD PET in a translational model, which overall support the method applicability when evaluating novel cardio-protective therapies. |
| Databáze: | OpenAIRE |
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