Cryopreservation of Isolated Rat Hepatocytes: Effects of Iron-Mediated Oxidative Stress on Metabolic Activity
| Autor: | V. I. Grischenko, V A Koptelov, W. De Loecker, P De Loecker, Bj Fuller |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Aminoisobutyric Acids
Free Radicals Liver cytology Iron In Vitro Techniques Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Membrane Lipids chemistry.chemical_compound Malondialdehyde medicine Animals Isoleucine Rats Wistar Cryopreservation chemistry.chemical_classification General Medicine Metabolism Membrane transport Rats Amino acid Oxidative Stress medicine.anatomical_structure Liver Biochemistry chemistry Hepatocyte Lipid Peroxidation General Agricultural and Biological Sciences Oxidative stress |
| Zdroj: | Cryobiology. 34:150-156 |
| ISSN: | 0011-2240 |
| DOI: | 10.1006/cryo.1996.1993 |
| Popis: | In an attempt to quantitatively evaluate the destructive effects of free radicals on metabolism, freshly prepared and cryopreserved isolated rat hepatocytes were exposed to and incubated with Fe2+ compounds, reputedly inducing oxygen-derived free radicals (OFR) capable of attacking the lipid structures of cellular membranes. Malondialdehyde (MDA) formation was interpreted as an expression of free radical interaction with polyunsaturated lipids, and in vitro incubations were carried out during the period of constant MDA formation. Protein synthesizing activity was evaluated by incubating control hepatocytes and cells previously exposed to 100 microM of Fe2+, to 100 microM of Fe2+, and 100 microM of desferrioxamine and to 100 microM of desferrioxamine alone with 0.1 microCi of L-[U-14C]isoleucine and in the presence of these compounds. Membrane transport activity was similarly evaluated by following the cellular uptake of alpha-amino-[1-14C]isobutyric acid. Protein-synthesizing activity of freshly prepared and cryopreserved hepatocytes was not affected by Fe2+ treatment, nor by the additions of the iron chelator desferrioxamine. Amino acid transport, however, was inhibited by 100 microM of Fe2+, but was effectively neutralized by the simultaneous addition of 100 microM of desferrioxamine. Cryopreserved hepatocytes equally presented a significantly inhibited amino acid transport activity over the incubation period. The results suggest that the metabolic depression measured in thawed hepatocytes does not result to any large extent from iron-catalysed OFR effects. When OFR production was deliberately induced, the most significant early change was seen in transmembrane amino acid uptake in both fresh and cryopreserved cells. |
| Databáze: | OpenAIRE |
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