Induced responses to pathogen infection in Norway spruce phloem: changes in polyphenolic parenchyma cells, chalcone synthase transcript levels and peroxidase activity
| Autor: | Trygve Krekling, Paal Krokene, Anders Lönneborg, Nina Elisabeth Nagy, Carl Gunnar Fossdal, Halvor Solheim |
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| Rok vydání: | 2004 |
| Předmět: |
Chalcone synthase
Physiology Clone (cell biology) Plant Science Polymerase Chain Reaction Trees Microbiology Ascomycota Phenols Gene Expression Regulation Plant Parenchyma Picea Pathogen Peroxidase Plant Diseases Flavonoids Plant Stems biology Polyphenols Picea abies Pathogenic fungus Blotting Northern biology.organism_classification Biochemistry RNA Plant biology.protein Phloem Isoelectric Focusing Acyltransferases |
| Zdroj: | Tree Physiology. 24:505-515 |
| ISSN: | 1758-4469 0829-318X |
| DOI: | 10.1093/treephys/24.5.505 |
| Popis: | Polyphenolic parenchyma cells (PP cells) in Norway spruce (Picea abies (L.) Karst.) stem phloem play important roles in constitutive and inducible defenses. To determine whether anatomical and molecular changes in PP cells are correlated with tree resistance, we infected two Norway spruce clones with the pathogenic fungus Ceratocystis polonica (Siem.) C. Moreau. The fungus induced significantly different lesion lengths in the two clones, indicating that one clone was more resistant to the fungus (short lesions) than the other (long lesions). After infection, the cross-sectional area of PP cells and their vacuolar polyphenol bodies increased in the three most recent annual rings of PP cells in both clones. The more resistant clone had larger PP cells with denser polyphenol bodies than the less resistant clone, whereas the less resistant clone accumulated relatively more polyphenols after infection. Compared with the less resistant clone, the more resistant clone contained higher starch concentrations before infection that were reduced more quickly after infection before returning to original values. Low transcript levels of chalcone synthase were detected in uninfected tissues of both clones, but the levels increased dramatically after infection. Transcript levels were higher and peaked 6 days earlier in the more resistant clone than in the less resistant clone. The activity of at least one highly basic peroxidase isoform was greatly enhanced after infection, and this increase occurred earlier in the more resistant clone. |
| Databáze: | OpenAIRE |
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