Agrobacterium rhizogenes rolB gene affects photosynthesis and chlorophyll content in transgenic tomato (Solanum lycopersicum L.) plants
| Autor: | Fabiola Fani, Luigi Lazzara, P. Bettini, Lorenzo Melani, Massimiliano Marvasi, Maria Luisa Mauro, E. Cosi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Chlorophyll
0106 biological sciences 0301 basic medicine Agrobacterium rhizogenes rolB gene Agrobacterium Physiology Transgene Photosynthesis Suppression subtractive hybridization Tomato Agronomy and Crop Science Plant Science Photosynthetic efficiency Real-Time Polymerase Chain Reaction 01 natural sciences 03 medical and health sciences Solanum lycopersicum Gene Expression Regulation Plant Gene expression Botany Genetically modified tomato Gene Gene Library biology Gene Expression Profiling fungi Reproducibility of Results food and beverages Plants Genetically Modified biology.organism_classification Chloroplast Phenotype 030104 developmental biology Genes Bacterial Solanum 010606 plant biology & botany |
| ISSN: | 0176-1617 |
| Popis: | Insertion of Agrobacterium rhizogenes rolB gene into plant genome affects plant development, hormone balance and defence. However, beside the current research, the overall transcriptional response and gene expression of rolB as a modulator in plant is unknown. Transformed rolB tomato plant (Solanum lycopersicum L.) cultivar Tondino has been used to investigate the differential expression profile. Tomato is a well-known model organism both at the genetic and molecular level, and one of the most important commercial food crops in the world.\ud \ud Through the construction and characterization of a cDNA subtracted library, we have investigated the differential gene expression between transgenic clones of rolB and control tomato and have evaluated genes specifically transcribed in transgenic rolB plants. Among the selected genes, five genes encoding for chlorophyll a/b binding protein, carbonic anhydrase, cytochrome b6/f complex Fe-S subunit, potassium efflux antiporter 3, and chloroplast small heat-shock protein, all involved in chloroplast function, were identified. Measurement of photosynthesis efficiency by the level of three different photosynthetic parameters (Fv/Fm, rETR, NPQ) showed rolB significant increase in non-photochemical quenching and a, b chlorophyll content. Our results point to highlight the role of rolB on plant fitness by improving photosynthesis. |
| Databáze: | OpenAIRE |
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