Modulation of Rumen Microbes Through Extracellular Vesicle Released by the Rumen Fluke Calicophoron daubneyi
| Autor: | Toby Wilkinson, Aspen R Taylor-Mew, Helen Phillips, Russell M. Morphew, Nathan R. Allen, Peter M. Brophy, Sharon Huws |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Proteases Rumen 030231 tropical medicine Immunology Cattle Diseases Proteomics Microbiology Cathepsin L 03 medical and health sciences Extracellular Vesicles 0302 clinical medicine Cellular and Infection Microbiology proteomics Parasite hosting Animals Original Research mass spectrometry Differential centrifugation rumen microbiome biology Calicophoron daubneyi Extracellular vesicle biology.organism_classification QR1-502 030104 developmental biology Infectious Diseases Biochemistry biology.protein Cattle Trematoda extracellular vesicle |
| Zdroj: | Frontiers in Cellular and Infection Microbiology, Vol 11 (2021) Frontiers in Cellular and Infection Microbiology |
| ISSN: | 2235-2988 |
| DOI: | 10.3389/fcimb.2021.661830/full |
| Popis: | Parasite derived extracellular vesicles (EVs) have been proposed to play key roles in the establishment and maintenance of infection. Calicophoron daubneyi is a newly emerging parasite of livestock with many aspects of its underpinning biology yet to be resolved. This research is the first in-depth investigation of EVs released by adult C. daubneyi. EVs were successfully isolated using both differential centrifugation and size exclusion chromatography (SEC), and morphologically characterized though transmission electron microscopy (TEM). EV protein components were characterized using a GeLC approach allowing the elucidation of comprehensive proteomic profiles for both their soluble protein cargo and surface membrane bound proteins yielding a total of 378 soluble proteins identified. Notably, EVs contained Sigma-class GST and cathepsin L and B proteases, which have previously been described in immune modulation and successful establishment of parasitic flatworm infections. SEC purified C. daubneyi EVs were observed to modulate rumen bacterial populations by likely increasing microbial species diversity via antimicrobial activity. This data indicates EVs released from adult C. daubneyi have a role in establishment within the rumen through the regulation of microbial populations offering new routes to control rumen fluke infection and to develop molecular strategies to improve rumen efficiency. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|