The binding of animal low-density lipoproteins to human apolipoprotein(a)
| Autor: | V N Trieu, W J McConathy |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Genetically modified mouse
Apolipoprotein B Mice Transgenic CHO Cells Apoprotein(a) Biochemistry Mice Species Specificity Cricetinae Low density Animals Humans Cloning Molecular Molecular Biology Cells Cultured biology Chemistry Human apolipoprotein Disulfide bond nutritional and metabolic diseases Cell Biology Molecular biology Lipoproteins LDL Apolipoproteins Covalent bond biology.protein lipids (amino acids peptides and proteins) Rabbits Chickens Lipoprotein(a) Protein Binding Research Article Lipoprotein |
| Zdroj: | Biochemical Journal. 309:899-904 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3090899 |
| Popis: | Lipoprotein(a) [Lp(a)] is a risk factor for coronary artery disease. It is composed of lipids and apolipoprotein(a) [apo(a)] linked to apolipoprotein B (apoB) by a disulphide bond between Cys-4057 of apo(a)'s kringle 36 and possibly Cys-3734 of apoB. We call this the covalent apo(a): apoB-Lp interaction, to distinguish it from the non-covalent apo(a)/Lp(a): apoB-Lp interaction, which is probably mediated by apo(a)'s kringle 33 and residues 3304-3317 of apoB. The non-covalent interaction could be the initial interaction which brings apo(a) and apoB together prior to covalent linkage and Lp(a) formation. The non-covalent apo(a)/Lp(a)-binding site on apoB is evolutionarily more ancient than the covalent apo(a)-binding site on apoB. Both human and non-human low-density lipoproteins (LDLs) bind non-covalently to human apo(a)/Lp(a); however, only rabbit and human LDLs bind covalently to human apo(a). The non-covalent interaction between mouse LDL and human apo(a)/Lp(a) has a Kd of (1.7 +/- 1.33) x 10(-7) M (n = 3). This explains the co-localization of human apo(a) and mouse apoB in the atherosclerotic lesions of human apo(a) transgenic mice and supports our hypothesis that the non-covalent interaction is a contributing factor to apo(a) atherogenicity. |
| Databáze: | OpenAIRE |
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