A modified elliptical formula to estimate kidney collagen content in a model of chronic kidney disease

Autor: Jake A. Nieto, Itzhak D. Goldberg, Janice Zhu, Bin Duan, Michael A. Yamin, Ping Zhou, Latha Paka, Prakash Narayan, Jingsong Li
Rok vydání: 2017
Předmět:
Male
Pathology
Biopsy
030232 urology & nephrology
lcsh:Medicine
030204 cardiovascular system & hematology
urologic and male genital diseases
Kidney
Biochemistry
Diagnostic Radiology
Mice
0302 clinical medicine
Fibrosis
Chronic Kidney Disease
Ultrasound Imaging
Medicine and Health Sciences
lcsh:Science
Coloring Agents
Musculoskeletal System
Multidisciplinary
medicine.diagnostic_test
Radiology and Imaging
Organ Size
3. Good health
medicine.anatomical_structure
Nephrology
Physical Sciences
Collagen
Anatomy
Renal pelvis
Research Article
medicine.medical_specialty
Ellipsoids
Imaging Techniques
Geometry
Surgical and Invasive Medical Procedures
Research and Analysis Methods
Models
Biological
Pelvis
03 medical and health sciences
Diagnostic Medicine
Parenchyma
medicine
Animals
Humans
Renal Insufficiency
Chronic
business.industry
lcsh:R
Kidney metabolism
Biology and Life Sciences
Proteins
Kidneys
Renal System
medicine.disease
Disease Models
Animal
lcsh:Q
business
Azo Compounds
Collagens
Mathematics
Kidney disease
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 1, p e0190815 (2018)
ISSN: 1932-6203
Popis: The extent of scarring or renal interstitial collagen deposition in chronic kidney disease (CKD) can only be ascertained by highly invasive, painful and sometimes risky, tissue biopsy. Interestingly, while CKD-related abnormalities in kidney size can often be visualized using ultrasound, not only does the ellipsoid formula used today underestimate true renal size, but the calculated renal size does not inform tubulointerstitial collagen content. We used coronal kidney sections from healthy mice and mice with kidney disease to develop a new formula for estimating renal parenchymal area. While treating the kidney as an ellipse with the major axis (a) the polar distance, this technique involves extending the minor axis (b) into the renal pelvis to obtain a new minor axis, be. The calculated renal parenchymal area is remarkably similar to the true or measured area. Biochemically determined kidney collagen content revealed a strong and positive correlation with the calculated renal parenchymal area. Picrosirius red staining for tubulointerstitial collagen also correlated with calculated renal parenchymal area. The extent of renal scarring, i.e. kidney interstitial collagen content, can now be computed by making just two axial measurements which can easily be accomplished via noninvasive imaging of this organ.
Databáze: OpenAIRE
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