Immunocytochemical analysis of embryonic compartmentation with a monoclonal antibody against a cytokeratin-related antigen
| Autor: | L. Cleland, S. F. Gilbert, K. Brewer, G. B. Grunwald, M. Kawai |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Mesoderm
animal structures Histology Down-Regulation Fluorescent Antibody Technique Ectoderm Chick Embryo Germ layer Biology Retina Mice Antigen Antibody Specificity medicine Animals Antigens Molecular Biology Tissue Extracts Endoderm Embryogenesis Neural tube Antibodies Monoclonal Cell Differentiation Cell Biology General Medicine Immunohistochemistry Molecular biology Medical Laboratory Technology medicine.anatomical_structure Neurulation embryonic structures Keratins Anatomy General Agricultural and Biological Sciences Head Neural plate |
| Zdroj: | Histochemistry. 94:545-553 |
| ISSN: | 1432-119X 0301-5564 |
| DOI: | 10.1007/bf00272620 |
| Popis: | Mab 113F4, a monoclonal antibody recognizing an antigen in the outer synaptic layer of the chick neural retina, also recognizes an antigen appearing in all three germ layers of the gastrulating chick embryo. However, as neurulation proceeds, the antigen is down-regulated in three distinct patterns. First, the antigen is lost specifically from those trunk ectodermal cells destined to form the neural plate and, later, the neural tube. It remains absent from any neural derivative until day 13 when it appears in the outer synaptic layer of the neural retina, coincident with synaptogenesis in this region. Second, the entirety of the head ectoderm loses this antigen as the head lifts off the blastoderm. This down-regulation is followed later by a similar loss of antigen expression in the trunk ectoderm. Third, expression in the mesoderm becomes limited to the lateral plate and extraembryonic epithelia. Endodermal derivatives continue to express the antigen throughout development. Antigen 113F4 is localized within the cytoplasm and is organized in a fibrillar pattern. The intracellular localization of this antigen and its characteristic spatio-temporal tissue distribution are consistent with the antigen being a cytokeratin or cytokeratin-related antigen. The changes in tissue distribution suggest a possible role in tissue modelling in response to inductive interactions during development. |
| Databáze: | OpenAIRE |
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