Complete genome sequence and analysis of Alcaligenes faecalis strain Mc250, a new potential plant bioinoculant

Autor: Fernanda Carla Ferreira, Angélica Bianchini Sanchez, José S. L. Patané, Alessandro M. Varani, Washington Luiz Caneschi, Renata de Almeida Barbosa Assis, Aline Maria Da Silva, Érica Barbosa Felestrino, Leandro Marcio Moreira, Morghana Marina Villa, Jesus Aparecido Ferro, Leandro Grassi de Freitas, Natasha Peixoto Fonseca, Isabella Ferreira Cordeiro, Flávio Fonseca do Carmo, Luciana Hiromi Yoshino Kamino, Andrew Maltez Thomas, João C. Setubal, Camila Carrião Machado Garcia, Robson Soares Silva, Nalvo F. Almeida, Izadora Tabuso Vieira, Camila Gracyelle de Carvalho Lemes
Přispěvatelé: Universidade Federal de Ouro Preto, Universidade Federal de Ouro Preto (UFOP), Instituto Pristino, Universidade de São Paulo (USP), Universidade Federal de Viçosa (UFV), Universidade Estadual Paulista (Unesp), Universidade Federal de Mato Grosso do Sul (UFMS)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Citrus
Mimosa
Nematoda
Denitrification pathway
Gene Expression
Protein Synthesis
Ectoine
Genome
Biochemistry
Xanthomonas citri
chemistry.chemical_compound
Phylogeny
Protein Metabolism
Genetics
0303 health sciences
Multidisciplinary
Bacterial Genomics
Alcaligenes faecalis
Microbial Genetics
Chemical Synthesis
food and beverages
Drug Resistance
Microbial
Genomics
Anti-Bacterial Agents
Chemistry
Physical Sciences
Medicine
Metabolic Pathways
DNA
Circular
Research Article
Biosynthetic Techniques
Genomic Islands
Iron
Science
Microbial Genomics
Biology
Research and Analysis Methods
Microbiology
03 medical and health sciences
Phenols
BACTÉRIAS GRAM-NEGATIVAS
Metals
Heavy
Bacterial Genetics
Animals
Gene Regulation
Gene
030304 developmental biology
Whole genome sequencing
Base Sequence
Whole Genome Sequencing
030306 microbiology
Chemical Compounds
Biology and Life Sciences
Computational Biology
Proteins
Bacteriology
biology.organism_classification
Genome Analysis
Metabolism
chemistry
Genome
Bacterial
Zdroj: PLoS ONE, Vol 15, Iss 11, p e0241546 (2020)
PLoS ONE
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 1932-6203
Popis: Made available in DSpace on 2021-06-25T10:44:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-01 Here we present and analyze the complete genome of Alcaligenes faecalis strain Mc250 (Mc250), a bacterium isolated from the roots of Mimosa calodendron, an endemic plant growing in ferruginous rupestrian grasslands in Minas Gerais State, Brazil. The genome has 4,159,911 bp and 3,719 predicted protein-coding genes, in a single chromosome. Comparison of the Mc250 genome with 36 other Alcaligenes faecalis genomes revealed that there is considerable gene content variation among these strains, with the core genome representing only 39% of the protein-coding gene repertoire of Mc250. Mc250 encodes a complete denitrification pathway, a network of pathways associated with phenolic compounds degradation, and genes associated with HCN and siderophores synthesis; we also found a repertoire of genes associated with metal internalization and metabolism, sulfate/sulfonate and cysteine metabolism, oxidative stress and DNA repair. These findings reveal the genomic basis for the adaptation of this bacterium to the harsh environmental conditions from where it was isolated. Gene clusters associated with ectoine, terpene, resorcinol, and emulsan biosynthesis that can confer some competitive advantage were also found. Experimental results showed that Mc250 was able to reduce (∼60%) the virulence phenotype of the plant pathogen Xanthomonas citri subsp. citri when co-inoculated in Citrus sinensis, and was able to eradicate 98% of juveniles and stabilize the hatching rate of eggs to 4% in two species of agricultural nematodes. These results reveal biotechnological potential for the Mc250 strain and warrant its further investigation as a biocontrol and plant growth-promoting bacterium. Núcleo de Pesquisas em Ciências Biológicas (NUPEB) Universidade Federal de Ouro Preto Departamento de Ciências Biológicas (DECBI) Instituto de Ciências Exatas e Biológicas (ICEB) Universidade Federal de Ouro Preto (UFOP) Instituto Pristino Departamento de Bioquimica (DBQ) Instituto de Quimica (IQ) Universidade de São Paulo (USP) Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO) Universidade Federal de Vicosa (UFV) Departamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal (FCAV) Universidade Estadual Paulista (UNESP) Faculdade de Computação (FACOM) Universidade Federal de Mato Grosso do Sul Departamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal (FCAV) Universidade Estadual Paulista (UNESP)
Databáze: OpenAIRE
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