Contribution of molecular tools for the diagnosis and epidemiology of fungal chronic rhinosinusitis
| Autor: | Pauline Comacle, François Le gall, Sorya Belaz, Jean-Pierre Gangneux, Franck Jegoux, Florence Robert-Gangneux, Christophe Ruaux |
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| Přispěvatelé: | Service de Parasitologie-Mycologie [Rennes], Université de Rennes (UR)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Service d'ORL et de chirurgie maxillo-faciale [Rennes] = ENT Head and Neck Surgery [Rennes], CHU Pontchaillou [Rennes], Clinique mutualiste La Sagesse, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-CHU Pontchaillou [Rennes], Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Jonchère, Laurent |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Microbiological Techniques sequence analysis Chronic rhinosinusitis Direct examination Polymerase Chain Reaction Aspergillus fumigatus Hospitals University 0302 clinical medicine [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Epidemiology 030223 otorhinolaryngology DNA Fungal [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology Rhinitis Molecular Epidemiology biology Chronic sinusitis General Medicine Middle Aged 3. Good health Fungal sinusitis Infectious Diseases PCR ITS1/ITS2 Molecular Diagnostic Techniques [SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases Female Cladosporium medicine.medical_specialty 030106 microbiology Sensitivity and Specificity Microbiology Scedosporium 03 medical and health sciences Internal medicine DNA Ribosomal Spacer medicine otorhinolaryngologic diseases Humans Sinusitis [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs Retrospective Studies chronic rhinosinusitis Sequence Analysis DNA biology.organism_classification medicine.disease [SDV.MP.MYC] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology Mycoses Chronic Disease fungi |
| Zdroj: | Medical Mycology Medical Mycology, 2016, 54 (8), pp.794-800. ⟨10.1093/mmy/myw041⟩ Medical Mycology, Oxford University Press, 2016, 54 (8), pp.794-800. ⟨10.1093/mmy/myw041⟩ |
| ISSN: | 1369-3786 1365-280X |
| DOI: | 10.1093/mmy/myw041⟩ |
| Popis: | International audience; Chronic rhinosinusitis (CRS) rank second at chronic inflammatory diseases in industrialized countries and are an important public health concern. Diagnosis relies on a set of arguments including clinical signs, imaging, histopathologic and mycological analyses of sinus specimens, collected during nasal endoscopy. The sensitivity of fungal cultures is reported to be poor, even when direct examination is positive, thus the epidemiology of fungal chronic sinusitis is ill-known. This study evaluated the sensitivity of molecular diagnosis in 70 consecutive samples (61 patients with CRS) analysed at the University Hospital of Rennes during a 3-year period. DNA detection was performed using a conventional PCR method targeting the ITS1/ITS2 sequence and the resulting amplification products were sequenced. Fungal CRS was proven in 42 patients (69%), of which only 20 (48%) had a positive culture. 37/42 (88%) patients were diagnosed with a fungus ball, 3 with allergic fungal CRS and 2 with undetermined fungal CRS. PCR was positive in all 42 cases and direct sequencing allowed to identify fungi in all cases but one, and detected multiple infection in 3. Aspergillus fumigatus was present in 69% of patients; Cladosporium cladosporoides in 9.5%, Scedosporium sp., A. nidulans and A. flavus in 7% each. In 2/19 patients with negative direct examination, sequencing analysis revealed the presence of Capnobotryella sp. and C. cladosporoides, in clinical settings compatible with fungal sinusitis. In conclusion, ITS1/ITS2 PCR had a twice better sensitivity than culture, and combined sequencing provides accurate epidemiological data on fungal CRS. |
| Databáze: | OpenAIRE |
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