Genetic diversity of mtDNA D-loop sequences in four native Chinese chicken breeds
| Autor: | Xuelian Liu, Ruili Han, Zhuanjian Li, Xiangtao Kang, Guoxi Li, H W Guo, Cuicui Li, Xinlei Wang, G R Sun |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mitochondrial DNA China animal structures Biology medicine.disease_cause DNA Mitochondrial Haplogroup Nucleotide diversity 03 medical and health sciences D-loop Polymorphism (computer science) medicine Animals Phylogeny Genetics Mutation Genetic diversity Base Sequence Haplotype 0402 animal and dairy science Genetic Variation 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science 030104 developmental biology Haplotypes embryonic structures Animal Science and Zoology Female Chickens Food Science |
| Zdroj: | British poultry science. 58(5) |
| ISSN: | 1466-1799 |
| Popis: | 1. To explore the genetic diversity of Chinese indigenous chicken breeds, a 585 bp fragment of the mitochondrial DNA (mtDNA) region was sequenced in 102 birds from the Xichuan black-bone chicken, Yunyang black-bone chicken and Lushi chicken. In addition, 30 mtDNA D-loop sequences of Silkie fowls were downloaded from NCBI. The mtDNA D-loop sequence polymorphism and maternal origin of 4 chicken breeds were analysed in this study. 2. The results showed that a total of 33 mutation sites and 28 haplotypes were detected in the 4 chicken breeds. The haplotype diversity and nucleotide diversity of these 4 native breeds were 0.916 ± 0.014 and 0.012 ± 0.002, respectively. Three clusters were formed in 4 Chinese native chickens and 12 reference breeds. Both the Xichuan black-bone chicken and Yunyang black-bone chicken were grouped into one cluster. Four haplogroups (A, B, C and E) emerged in the median-joining network in these breeds. 3. It was concluded that these 4 Chinese chicken breeds had high genetic diversity. The phylogenetic tree and median network profiles showed that Chinese native chickens and its neighbouring countries had at least two maternal origins, one from Yunnan, China and another from Southeast Asia or its surrounding area. |
| Databáze: | OpenAIRE |
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