MicroRNA-125b Functions as a Tumor Suppressor in Hepatocellular Carcinoma Cells
| Autor: | Shi-Ming Wang, Huiyu Li, Yu-Xuan Wang, Yan-Zhang Tian, Hao-Liang Zhao, Hongyan Jia, Wen-Ting Yan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Carcinoma Hepatocellular Biology hepatocellular carcinoma miR-125b Mcl-1 IL6R medicine.disease_cause Catalysis Article lcsh:Chemistry Inorganic Chemistry microRNA medicine Humans Genes Tumor Suppressor Northern blot RNA Neoplasm Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology neoplasms Spectroscopy Cell growth Reverse Transcriptase Polymerase Chain Reaction Organic Chemistry Cell Cycle Liver Neoplasms Cancer General Medicine Hep G2 Cells Cell cycle medicine.disease Molecular biology Receptors Interleukin-6 digestive system diseases Computer Science Applications Gene Expression Regulation Neoplastic MicroRNAs lcsh:Biology (General) lcsh:QD1-999 Cell culture Cancer research Myeloid Cell Leukemia Sequence 1 Protein Ectopic expression Female Carcinogenesis |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 13, Iss 7, Pp 8762-8774 (2012) International Journal of Molecular Sciences; Volume 13; Issue 7; Pages: 8762-8774 |
| ISSN: | 1422-0067 |
| Popis: | MicroRNAs (miRNAs) are important regulators of multiple cellular processes, and the deregulation of miRNA is a common event in diverse human diseases, particularly cancer. However, the mechanisms underlying the relationship between disordered miRNA expression and tumorigenesis have remained largely unknown. In this study, we demonstrated the down-regulation of miR-125b in hepatocellular carcinoma (HCC) tissues and HCC cell lines by Northern blot and quantitative RT-PCR analyses. The ectopic expression of miR-125b reduced the cellular proliferation and cell cycle progression of HCC cells by targeting Mcl-1 and IL6R. Furthermore, the miR-125b-induced inhibition of cell proliferation was rescued by the expression of Mcl-1 or IL6R variants that lacked 3' UTRs. Thus, this study revealed the differential expression of miR-125b in HCC cells and elucidated its potential as a tumor suppressor in HCC development. |
| Databáze: | OpenAIRE |
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