Optimization of Wide-Field ODMR Measurements Using Fluorescent Nanodiamonds to Improve Temperature Determination Accuracy
| Autor: | Wataru Kada, Ryuji Igarashi, Osamu Hanaizumi, Kiichi Kaminaga, Tamami Yanagi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science General Chemical Engineering Monte Carlo method 02 engineering and technology Temperature measurement Sweep frequency response analysis lcsh:Chemistry 03 medical and health sciences nitrogen-vacancy centers General Materials Science fluorescent nanodiamonds 030304 developmental biology 0303 health sciences business.industry Communication Quantum sensor Time resolution 021001 nanoscience & nanotechnology Wide field Fluorescence nanometer-scale thermometry lcsh:QD1-999 Optoelectronics 0210 nano-technology business |
| Zdroj: | Nanomaterials, Vol 10, Iss 2282, p 2282 (2020) Nanomaterials |
| ISSN: | 2079-4991 |
| Popis: | Fluorescent nanodiamonds containing nitrogen-vacancy centers have attracted attention as nanoprobes for temperature measurements in microenvironments, potentially enabling the measurement of intracellular temperature distributions and temporal changes. However, to date, the time resolution and accuracy of the temperature determinations using fluorescent nanodiamonds have been insufficient for wide-field fluorescence imaging. Here, we describe a method for highly accurate wide-field temperature imaging using fluorescent nanodiamonds for optically detected magnetic resonance (ODMR) measurements. We performed a Monte Carlo simulation to determine the optimal frequency sweep range for ODMR temperature determination. We then applied this sweep range to fluorescent nanodiamonds. As a result, the temperature determination accuracies were improved by a factor ~1.5. Our result paves the way for the contribution of quantum sensors to cell biology for understanding, for example, differentiation in multicellular systems. |
| Databáze: | OpenAIRE |
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