A guide for an anatomically sensitive dentine microsampling and age-alignment approach for human teeth isotopic sequences
| Autor: | Julia A. Lee-Thorp, Andrea Czermak, Teresa Fernández-Crespo, Peter Ditchfield |
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| Přispěvatelé: | School of Archaeology, University of Oxford, Laboratoire méditerranéen de préhistoire Europe-Afrique (LAMPEA), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), School of Archaeology [Oxford], University of Oxford, Agence Nationale de la Recherche, Grant/Award Number: DFG246602782, British Academy (Newton International Fellowship), Grant/Award Number: NF170854, Deutsche Forschungsgemeinschaft, Grant/Award Number: DFG246602782, European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement, Grant/Award Number: 790491, John Fell Fund, University of Oxford, Grant/Award Number: EBD10940-151/102., Fernandez-Crespo, Teresa |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Adolescent [SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory Tertiary dentine [SHS.ANTHRO-BIO]Humanities and Social Sciences/Biological anthropology Incremental growth Anthropology Physical Young Adult stomatognathic system medicine Humans Cementum Child ComputingMilieux_MISCELLANEOUS Carbon Isotopes [SHS.ARCHEO] Humanities and Social Sciences/Archaeology and Prehistory Nitrogen Isotopes Infant [SHS.ANTHRO-BIO] Humanities and Social Sciences/Biological anthropology Diet stomatognathic diseases medicine.anatomical_structure [SHS.HIST] Humanities and Social Sciences/History Child Preschool Anthropology Temporal resolution Dentin Secondary dentine Age Determination by Teeth Anatomy [SHS.HIST]Humanities and Social Sciences/History Tooth Geology Biomedical engineering |
| Zdroj: | American Journal of Physical Anthropology American Journal of Physical Anthropology, Wiley, 2020 American Journal of Physical Anthropology, 2020, 173 (4), pp.776-783. ⟨10.1002/ajpa.24126⟩ |
| ISSN: | 0002-9483 1096-8644 |
| DOI: | 10.1002/ajpa.24126⟩ |
| Popis: | International audience; ObjectivesStable isotope analysis of sequential dentine samples is a potentially powerful method to reveal insights into early life-histories of individuals in the past. Dentine incremental growth structures are complex, however, and current approaches that apply horizontal sectioning of demineralized tooth halves or quarters risk combining multiple growth layers and may include unwanted cementum or secondary dentine. They also require destruction of large parts of a tooth. Here, we present a less destructive and relatively straightforward protocol that reduces damage, increases temporal resolution, and improves the accuracy of age-alignment between individuals.Material and methodsWe outline a protocol that includes the sampling of small (1 mm diameter) cylindrical plug transects from a thin section, along with an age-alignment scheme predicated on average growth rates for dentine areas.Results and discussionThe proposed protocol is readily applicable and more anatomically sensitive than horizontal slicing. Micro-samples are smaller (in both length and depth), hence minimizing temporal overlap and avoid directions that may contravene growth pattern. They completely avoid areas where secondary and tertiary dentine or cementum can be deposited. Age-alignment is improved by using growth ratios of anatomical tooth zones.ConclusionThis method minimizes destruction, enables finer temporal resolution and facilitates data comparison. It can be readily combined with fluorescence imaging-based or other pre-screening methods of dentine collagen preservation. |
| Databáze: | OpenAIRE |
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