Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups
| Autor: | Aydın Tunçay, Ömer Naci Emiroğulları, Ecmel Mehmetbeyoglu, Deniz Elcik, Serpil Taheri, Işın Güneş, Elif Funda Sener, Reyhan Tahtasakal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
RD1-811
Ischemia Apoptosis 030204 cardiovascular system & hematology RNA Messenger Caspase 9 Real-Time Polymerase Chain Reaction Andrology 03 medical and health sciences 0302 clinical medicine Gene expression medicine Autophagy Humans Diseases of the circulatory (Cardiovascular) system RNA Messenger Cardiac Surgical Procedures Vein Hypoxia Cardioplegic Solutions Caspase Whole blood Messenger RNA Aspartic Acid biology business.industry General Medicine Hypoxia (medical) medicine.disease medicine.anatomical_structure RC666-701 biology.protein Heart Arrest Induced Original Article Heart Arrest Induced Surgery medicine.symptom Cardiology and Cardiovascular Medicine business |
| Zdroj: | Brazilian Journal of Cardiovascular Surgery, Vol 36, Iss 3, Pp 331-337 (2021) Brazilian Journal of Cardiovascular Surgery Brazilian Journal of Cardiovascular Surgery v.36 n.3 2021 Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) instacron:SBCCV Brazilian Journal of Cardiovascular Surgery, Volume: 36, Issue: 3, Pages: 331-337, Published: 05 MAR 2021 |
| ISSN: | 1678-9741 |
| Popis: | Introduction: Blood cardioplegia (BC) and Custodiol cardioplegia (CC) have been used for a long time in open heart surgery and are highly effective solutions. The most controversial issue among these two is whether there is any difference between them regarding myocardial damage after ischemia surgery. In this study, autophagy, apoptosis, and hypoxia markers were investigated and that way we evaluated the differences between BC and CC patients. Methods: A total of 30 patients were included in this study, using two different cardioplegic solutions. Three different whole blood samples of the patients were taken from a central vein (preoperatively, immediately postoperatively, and one day after surgery). Total ribonucleic acid was extracted from these samples. Quantitative real-time polymerase chain reaction was performed, and changes in gene expression were determined by the 2-∆∆Ct method of relative quantification. Results: In the CC group, Beclin gene expression level was found to be higher and this difference was statistically significant (P=0.0024). Similarly, cysteine-aspartic acid protease (caspase) 9 and hypoxia-inducible factor 1α messenger ribonucleic acid (mRNA) gene expression level increased and were significantly different in the CC group. In the BC group, Beclin and microtubule-associated protein light chain 3 expressions were higher in the samples taken one day after surgery. Caspases 3 and 8 gene expressions were significantly different in the BC group. Conclusion: As a result of the analysis performed between the two cardioplegia groups, it has been shown that CC harms the myocardium more than BC at the level of mRNA expression of related markers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|