In vitro follicle culture shows that progestagens are the maturation-inducing hormones (MIH) and possible regulators of the ovulation-mediating hormone PGE2 in female Eurasian perch Perca fluviatilis
| Autor: | Sylvain Milla, Rose Bulteau, Pascal Fontaine, Sirine Selmi, Céline Chevalier, Leila El Mohajer |
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| Rok vydání: | 2020 |
| Předmět: |
Ovulation
endocrine system Physiology media_common.quotation_subject Aquatic Science Biochemistry Dinoprostone Human chorionic gonadotropin Andrology 03 medical and health sciences Follicle Ovarian Follicle medicine Animals Sexual Maturation Incubation Testosterone 030304 developmental biology media_common 0303 health sciences Perch biology 04 agricultural and veterinary sciences General Medicine biology.organism_classification Oocyte Hormones medicine.anatomical_structure Perches 040102 fisheries 0401 agriculture forestry and fisheries Female Hormone |
| Zdroj: | Fish physiology and biochemistry. 47(4) |
| ISSN: | 1573-5168 |
| Popis: | In European aquaculture, Eurasian perch, Perca fluviatilis L., is perceived as one of the most highly valuable freshwater fish species and a strong candidate for the development of freshwater aquaculture. In the pursuit of improving the quality of reproduction in this domesticated species, investigating the hormones mediating the final oocyte maturation (FOM) is therefore indispensable. But, the exact nature of the maturation-inducing hormone (MIH) in Eurasian perch is unknown. To further validate the existence of a maturation-inducing activity behind potential hormonal candidates in this species, we in vitro tested a group of nine hormones: cortisol (Co), 11-deoxycortisol (11-D), corticosterone (coS), 11-deoxycorticosterone (DOC), 17α,20βdihydroxy-4-pregnen-3-one (DHP) and 17α,20β,21 trihydroxy-4-pregnen-3-one (THP), prostaglandin E2 (PGE2), estradiol-17β (E2) and testosterone (T), in their ability to trigger FOM advancement and the production of sex steroids potentially involved in FOM. Using mature female perch, two in vitro experiments were conducted with oocytes at the start of the FOM. The follicles were incubated for 62 h in Cortland media with and without human chorionic gonadotropin (hCG). By the end of the incubation, only DHP and THP triggered the full advancement in FOM even at low doses with the effect of DHP being in vivo validated. However, the de novo productions of E2 and DHP were not shown to be regulated by either of the MIH candidates. Progestagens are hence more credible candidates as MIH than corticosteroids in Eurasian perch. Our in vitro study also revealed that both PGE2 and DHP are strongly associated with ovulation and that PGE2 might have slightly contributed to such DHP activity. |
| Databáze: | OpenAIRE |
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