Effect of ErbB2 Coexpression on the Kinetic Interactions of Epidermal Growth Factor with Its Receptor in Intact Cells
| Autor: | James V. Staros, John C. Wilkinson |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Cell Survival
Cell Cell Culture Techniques Ligands Transfection Kinetic energy Biochemistry Fluorescence Cell Line Mice Transformation Genetic Epidermal growth factor medicine Animals Rapid mixing Receptor Epidermal Growth Factor Chemistry Cell Membrane Genes erbB-2 Molecular biology Recombinant Proteins ErbB Receptors Kinetics medicine.anatomical_structure Cell culture Biophysics Anisotropy hormones hormone substitutes and hormone antagonists Fluorescence anisotropy Signal Transduction |
| Zdroj: | Biochemistry. 41:8-14 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi015839l |
| Popis: | We have extended the use of stopped-flow mixing and fluorescence anisotropy detection to investigate in real-time the effects of ErbB2 coexpression on the kinetic interactions of epidermal growth factor (EGF) with the EGF receptor. Using stable 32D-derived cell lines expressing both the EGF receptor and ErbB2, and fluorescein-labeled H22Y murine EGF (F-EGF), a series of association and dissociation experiments were performed in which the kinetic interaction of F-EGF with cells was monitored by observing time-dependent changes in fluorescence anisotropy following rapid mixing. Data were collected at various concentrations of F-EGF and multiple cell densities, using cells that express similar levels of the EGF receptor but different levels of ErbB2, and then analyzed by fitting to a two independent receptor-class model using global analysis techniques. The recovered kinetic parameters indicated that the coexpression of ErbB2 had relatively modest effects on recovered rate constants and calculated K(d) values, but a significant effect on the fraction of receptors associated with the high-affinity receptor class. This effect on the fraction of high-affinity receptors depended on the relative expression of ErbB2, as higher ErbB2 expression levels correlated with a larger fraction of high-affinity receptors. Further, the increase in the fraction of high-affinity receptors due to the presence of ErbB2 occurred without any change in the total number of EGF binding sites per cell. Thus, we have identified modulation of the relative populations of high- and low-affinity classes of EGF receptors as a consequence of coexpression of ErbB2 with the EGF receptor. |
| Databáze: | OpenAIRE |
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