Cell-mediated immune response associated with Chlamydia pneumoniae infection in atherosclerotic patients
| Autor: | Fouzia Radouani, Fouad Seghrouchni, Loubna El Yazouli, Mohamed Bouazza, Aziz Aroussi Alami, Nadia Dakka, Hicham Hejaji |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Genotype CD8 Antigens CD14 030106 microbiology Lipopolysaccharide Receptors Inflammation Microbiology Peripheral blood mononuclear cell Flow cytometry 03 medical and health sciences Immune system Antigen Humans Medicine Chlamydophila Infections Immunity Cellular Chlamydia medicine.diagnostic_test business.industry Thrombosis Chlamydophila pneumoniae Atherosclerosis Flow Cytometry medicine.disease 030104 developmental biology Infectious Diseases CD4 Antigens Immunology Leukocytes Mononuclear medicine.symptom business CD8 |
| Zdroj: | Microbial Pathogenesis. 139:103860 |
| ISSN: | 0882-4010 |
| Popis: | Background Chlamydia pneumoniae is an obligate intracellular bacterium that activates cell mediated immune responses; several investigations have demonstrated its strong implication in atherosclerosis. Objectives The main objective of our study was to explore the cell-mediated immune response to C. pneumoniae infection in patients with atherosclerosis by evaluating CD14, CD8 and CD4 expression. Methods This investigation involved a total of 27 patients with atherosclerosis and 32 controls, among patients recruited to evaluate the association of C. pneumoniae with atherosclerosis. C. pneumoniae DNA was detected in PBMCs by nested PCR as described in our previous studies. CD4, CD8 and CD14 expression was measured by flow cytometry and data analysis was performed using FlowJo software. Results The results revealed an increase in MFI expression of CD4, CD8 and CD14 in Cpn DNA+ subjects among both patients and healthy subject controls (CD4 Cpn DNA+ = 829.11 vs. CD4 Cpn DNA- = 571.14; CD8 Cpn DNA+ = 1562 vs. CD8 Cpn DNA- = 699; CD14 Cpn DNA+ = 1513.83 vs. CD14 Cpn DNA- = 1170.70), with a statistically significant difference (p Conclusion These data provide incentive to further explore the role of C. pneumoniae in stimulating and changing mechanisms of the cell-mediated immune response induced by C. pneumoniae antigens. This may alter immune cell-mediated responses via increased expression of CD4, CD8 and CD14 during inflammation and the development of thrombosis, leading to fatal atherosclerosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect