Effects of topical prostaglandin drops on angiogenesis in an in ovo chick chorioallantoic membrane model
| Autor: | Mehmet Cem Sabaner, Ayhan Vurmaz, Reşat Duman, Tolga Ertekin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Vascular Endothelial Growth Factor A
animal structures Angiogenesis Prostaglandin Chick Embryo Pharmacology Toxicology In ovo Chorioallantoic Membrane Chick chorioallantoic membrane 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound Travoprost 0302 clinical medicine medicine Animals Humans Latanoprost Antihypertensive Agents Neovascularization Pathologic Bimatoprost Glaucoma General Medicine Chorioallantoic membrane chemistry Models Animal embryonic structures 030221 ophthalmology & optometry Ophthalmic Solutions medicine.drug |
| Zdroj: | Cutaneous and Ocular Toxicology. 40:54-60 |
| ISSN: | 1556-9535 1556-9527 |
| Popis: | To investigate the effects of bimatoprost, latanoprost and travoprost on angiogenesis in the chick chorioallantoic membrane (CAM) model in ovo.Fifty fertilized specific-pathogen-free chick eggs were used in this preclinical, prospective, experimental embryo study. Eggs were randomly distributed into 5 groups of ten eggs. Eggs were placed in the incubator after disinfection of their shells with alcohol and monitored appropriate temperature and humidity. On the 3rd day of incubation, a small window was opened on the eggshell. Bimatoprost in group 1, latanoprost in group 2, travoprost in group 3, bevacizumab in group 4, phosphate-buffered-saline (PBS) used in group 5 was applied by injection to CAM. The sterile film was glued onto the broken part of the shell and the eggs were placed in the incubator again. On the 8th day of incubation, eggs were opened and vascular structures on CAMs were examined. Digital photographs were taken, analysed in the ImageJ open source image processing software and differences between groups were evaluated. Thereafter, VEGF (Vascular endothelial growth factor) levels were measured appropriately in the embryo samples.All embryos in the prostaglandin groups and the PBS control group were observed to have life signs confirmed by heart rate. In 8 embryos in the bevacizumab group, no life signs were confirmed, while 2 embryos with life signs showed severe hypoplasia. Vascular density, number of vessels and VEGF levels in the bimatoprost, latanoprost and travoprost groups, there were statistically significantly higher than the PBS control group.This study demonstrates that topical prostaglandin drops increase angiogenesis in the chick CAM model in ovo. |
| Databáze: | OpenAIRE |
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