VAMP8 and serotonin transporter levels are associated with venous thrombosis risk in a Spanish female population. Results from the RETROVE Project
| Autor: | Dolors Llobet, Cristina Vallvé, Jordi Fontcuberta, Juan Souto, Marina Carrasco, Noelia Vilalta, Artur Oliver, Biel Cuevas, Joaquín Murillo, Lidia Román, José Mateo, Isabel Tirado |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
030204 cardiovascular system & hematology Logistic regression Gastroenterology R-SNARE Proteins 03 medical and health sciences 0302 clinical medicine Risk Factors Internal medicine Humans Medicine Platelet Serotonin transporter Serotonin Plasma Membrane Transport Proteins Venous Thrombosis biology business.industry Hematology Middle Aged medicine.disease Thrombosis Venous thrombosis Real-time polymerase chain reaction Spain 030220 oncology & carcinogenesis biology.protein Female Serotonin business Body mass index |
| Zdroj: | Thrombosis Research. 181:99-105 |
| ISSN: | 0049-3848 |
| Popis: | Introduction Platelet hyper-reactivity has been associated with thrombosis and high levels of human vesicle-associated membrane protein 8 (VAMP8) and serotonin transporter (SERT). Two polymorphisms (rs1010 of VAMP8 gene and in SERT gene (SLC6A4)) are associated with arterial thrombosis. Aim To determine if levels of serotonin, SERT and/or VAMP8 and these polymorphisms are associated with the risk of venous thrombosis. Material and methods A total of 324 individuals were included in the RETROVE Study (Riesgo de Enfermedad TROmboembolica VEnosa). VAMP8, SERT and serotonin were determined by ELISA; polymorphisms of SLC6A4 and VAMP8 by polymerase chain reaction (PCR) and real time PCR. The venous thrombotic risk was calculated by a logistic regression method to estimate the crude and adjusted OR (adjusted for sex, age, body mass index and venous thrombosis risk co-factors). Results Statistically significant high levels of VAMP8 and SERT were found in patients, but not in controls. In contrast, serotonin showed lower levels in patients than in controls. When individuals were studied by gender, only women exhibited a statistically significant difference: the OR for VAMP8 was 3.25 (1.61–6.56 95% CI). The adjusted OR did not change. The OR for SERT was 2.76 (1.36–5.60 95% CI), the adjusted OR was maintained also. For serotonin with OR of 2.62 (1.40–4.92 95% CI), the adjusted OR was not significant. In contrast males did not show significant differences. No statistically differences between patients and controls were found for both polymorphisms. Conclusions VAMP8 and SERT levels are associated with venous thrombosis in a female Spanish population. |
| Databáze: | OpenAIRE |
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