Impact of ischemia time on islet isolation success and posttransplantation outcomes: A retrospective study of 452 pancreas isolations

Autor: Sandrine Lablanche, Sophie Borot, Vanessa Lavallard, Estelle Brioudes, Fanny Lebreton, Géraldine Parnaud, Fanny Buron, Nadine Pernin, Charles-Henri Wassmer, Quentin Perrier, Ekaterine Berishvili, Kevin Bellofatto, Laurence Kessler, Anne Wojtusciszyn, Christophe Combescure, Domenico Bosco, David Cottet-Dumoulin, Thierry Berney
Přispěvatelé: Hôpitaux Universitaires de Genève (HUG), Université de Genève (UNIGE), Ilia State University [Tbilisi], CHU Grenoble, CHU Strasbourg, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg (UNISTRA), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)
Rok vydání: 2021
Předmět:
donors and donation
Islets of Langerhans Transplantation
Ischemic time
030230 surgery
Cold Ischemia Time
0302 clinical medicine
Endocrinology/diabetology
Ischemia
islet isolation
Retrospective analysis
Immunology and Allergy
Pharmacology (medical)
type 1 [Diabetes]
Islet transplantation
ddc:618
geography.geographical_feature_category
ddc:617
MESH: Pancreas
Islet
medicine.anatomical_structure
diabetes: type 1
Clinical research/practice
Cardiology
endocrinology/diabetology
islets of Langerhans
Pancreas
medicine.medical_specialty
Isolation (health care)
Organ Preservation Solutions
[SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery
clinical research/practice
Donors and donation
Islets of Langerhans
03 medical and health sciences
Insulin/C−peptide
Internal medicine
medicine
Humans
cardiovascular diseases
Retrospective Studies
Transplantation
geography
Islet isolation
MESH: Humans
business.industry
islet transplantation
MESH: Islets of Langerhans
MESH: Retrospective Studies
Retrospective cohort study
medicine.disease
MESH: Islets of Langerhans Transplantation
insulin/C−peptide
MESH: Ischemia
MESH: Organ Preservation Solutions
business
Zdroj: American Journal of Transplantation
American Journal of Transplantation, Wiley, 2021, 21 (4), pp.1493-1502. ⟨10.1111/ajt.16320⟩
American journal of transplantation, Vol. 21, No 4 (2021) pp. 1493-1502
ISSN: 1600-6135
1600-6143
DOI: 10.1111/ajt.16320
Popis: International audience; Many variables impact islet isolation, including pancreas ischemia time. The ischemia time upper limit that should be respected to avoid a negative impact on the isolation outcome is not well defined. We have performed a retrospective analysis of all islet isolations in our center between 2008 and 2018. Total ischemia time, cold ischemia time, and organ removal time were analyzed. Isolation success was defined as an islet yield ≥200 000 IEQ. Of the 452 pancreases included, 288 (64%) were successfully isolated. Probability of isolation success showed a significant decrease after 8 hours of total ischemia time, 7 hours of cold ischemia time, and 80 minutes of organ removal time. Although we observed an impact of ischemia time on islet yield, a probability of isolation success of 50% was still present even when total ischemia time exceeds 12 hours. Posttransplantation clinical outcomes were assessed in 32 recipients and no significant difference was found regardless of ischemia time. These data indicate that although shorter ischemia times are associated with better islet isolation outcomes, total ischemia time >12 hours can provide excellent results in appropriately selected donors.
Databáze: OpenAIRE
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