Epithelial Ovarian Cancer Diagnosis of Second-Harmonic Generation Images: A Semiautomatic Collagen Fibers Quantification Protocol
| Autor: | Victor Hugo Casco, Fátima Böttcher-Luiz, Carlos L. Cesar, Johana Sj Luna, Liliana Ala Andrade, Hernades F Carvahlo, Luciana Ariadna Erbes, Kynthia Sanchez Salas, Javier Adur, Angel A. Zeitoune |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Pathology medicine.medical_specialty SHG MICROSCOPY HUMAN CANCER OVARY Otras Ciencias Biológicas Malignancy 01 natural sciences lcsh:RC254-282 FFT 010309 optics purl.org/becyt/ford/1 [https] Microscopia Ciencias Biológicas 03 medical and health sciences Ovarian epithelial cancer 0103 physical sciences Biopsy Medicine DIAGNOSTIC PROTOCOL Epithelial ovarian cancer purl.org/becyt/ford/1.6 [https] Original Research diagnostic protocol medicine.diagnostic_test Neoplasias ovarianas business.industry human cancer ovary Second-harmonic generation Anisotropia GLCM medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Nonlinear optical microscopy Serous fluid 030104 developmental biology Oncology SHG microscopy Improvement methods Colágeno business CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Cancer Informatics Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC Cancer Informatics, Vol 16 (2017) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1176-9351 |
| Popis: | A vast number of human pathologic conditions are directly or indirectly related to tissular collagen structure remodeling. The nonlinear optical microscopy second-harmonic generation has become a powerful tool for imaging biological tissues with anisotropic hyperpolarized structures, such as collagen. During the past years, several quantification methods to analyze and evaluate these images have been developed. However, automated or semiautomated solutions are necessary to ensure objectivity and reproducibility of such analysis. This work describes automation and improvement methods for calculating the anisotropy (using fast Fourier transform analysis and the gray-level co-occurrence matrix). These were applied to analyze biopsy samples of human ovarian epithelial cancer at different stages of malignancy (mucinous, serous, mixed, and endometrial subtypes). The semiautomation procedure enabled us to design a diagnostic protocol that recognizes between healthy and pathologic tissues, as well as between different tumor types. Fil: Zeitoune, Angel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina Fil: Luna, Johana S. J.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina Fil: Sanchez Salas, Kynthia. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina Fil: Erbes, Luciana Ariadna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina Fil: Cesar, Carlos L.. Universidade Federal do Ceará; Brasil. National Institute of Science and Technology on Photonics Applied to Cell Biology; Brasil Fil: Andrade, Liliana A. L. A.. Universidade Estadual de Campinas; Brasil Fil: Carvahlo, Hernades F.. Universidade Estadual de Campinas; Brasil. National Institute of Science and Technology on Photonics Applied to Cell Biology; Brasil Fil: Bottcher Luiz, Fátima. Universidade Estadual de Campinas; Brasil. National Institute of Science and Technology on Photonics Applied to Cell Biology; Brasil Fil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina Fil: Adur, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina |
| Databáze: | OpenAIRE |
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