Soluble factors secreted by differentiating embryonic stem cells stimulate exogenous cell proliferation and migration
| Autor: | Christian Mandrycky, James C Waring, Marissa T Cooke, Alyssa V. Ngangan, Todd C. McDevitt |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Vascular Endothelial Growth Factor A
Somatic cell Cellular differentiation Medicine (miscellaneous) Bone Morphogenetic Protein 4 Embryoid body Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Mice 03 medical and health sciences Paracrine signalling 0302 clinical medicine Cell Movement Human Umbilical Vein Endothelial Cells Animals Humans Embryoid Bodies Cell Proliferation 030304 developmental biology 0303 health sciences Epidermal Growth Factor Cell growth Research Cell Differentiation 3T3 Cells Cell Biology Fibroblasts Embryonic stem cell Cell biology Vascular endothelial growth factor A Culture Media Conditioned Molecular Medicine Stem cell 030217 neurology & neurosurgery |
| Zdroj: | Stem Cell Research & Therapy |
| ISSN: | 1757-6512 |
| DOI: | 10.1186/scrt415 |
| Popis: | Introduction: Stem cells are being investigated as catalysts of tissue regeneration to either directly replace or promote cellularity lost as a result of traumatic injury or degenerative disease. In many reports, despite low numbers of stably integrated cells, the transient presence of cells delivered or recruited to sites of tissue remodeling globally benefits functional recovery. Such findings have motivated the need to determine how paracrine factors secreted from transplanted cells may be capable of positively impacting endogenous repair processes and somatic cell responses. Methods: Embryonic stem cells were differentiated as embryoid bodies (EBs) in vitro and media conditioned by EBs were collected at different intervals of time. Gene and protein expression analysis of several different growth factors secreted by EBs were examined by polymerase chain reaction and enzyme-linked immunosorbent assay analysis, respectively, as a function of time. The proliferation and migration of fibroblasts and endothelial cells treated with EB conditioned media was examined compared with unconditioned and growth media controls. Results: The expression of several growth factors, including bone morphogenic protein-4, insulin-like growth factors and vascular endothelial growth factor-A, increased during the course of embryonic stem cell (ESC) differentiation as EBs. Conditioned media collected from EBs at different stages of differentiation stimulated proliferation and migration of both fibroblasts and endothelial cells, based on 5-bromo-2′-deoxyuridine incorporation and transwell assays, respectively. Conclusions: Overall, these results demonstrate that differentiating ESCs express increasing amounts of various growth factors over time that altogether are capable of stimulating mitogenic and motogenic activity of exogenous cell populations. |
| Databáze: | OpenAIRE |
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