The RNA-binding protein HuR is a negative regulator in adipogenesis
| Autor: | Bryan C. Tan, Sook Yoong Chia, Yen Ching Lim, Arcinas Camille Esther Walet, Ufuk Degirmenci, Diana Teh Chee Siang, Lei Sun, Khaing Nwe Win, Xiang Hu, Dan Xu, Aung Maung Maung Kyaw |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Molecular biology Adipose Tissue White Science Regulator General Physics and Astronomy Repressor Adipose tissue White adipose tissue Biology General Biochemistry Genetics and Molecular Biology Article ELAV-Like Protein 1 03 medical and health sciences chemistry.chemical_compound Gene Knockout Techniques 0302 clinical medicine Endocrinology Adipose Tissue Brown Adipocyte Glucose Intolerance Developmental biology Animals Humans lcsh:Science Regulation of gene expression Inflammation Mice Knockout Gene knockdown Multidisciplinary Adipogenesis Intracellular Signaling Peptides and Proteins Membrane Proteins RNA-Binding Proteins Endocrine system and metabolic diseases General Chemistry Cell biology Mice Inbred C57BL 030104 developmental biology chemistry Adipose Tissue Gene Expression Regulation 030220 oncology & carcinogenesis lcsh:Q Insulin Resistance |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Human antigen R (HuR) is an essential regulator of RNA metabolism, but its function in metabolism remains unclear. This study identifies HuR as a major repressor during adipogenesis. Knockdown and overexpression of HuR in primary adipocyte culture enhances and inhibits adipogenesis in vitro, respectively. Fat-specific knockout of HuR significantly enhances adipogenic gene program in adipose tissues, accompanied by a systemic glucose intolerance and insulin resistance. HuR knockout also results in depot-specific phenotypes: it can repress myogenesis program in brown fat, enhance inflammation program in epidydimal white fat and induce browning program in inguinal white fat. Mechanistically, HuR may inhibit adipogenesis by recognizing and modulating the stability of hundreds of adipocyte transcripts including Insig1, a negative regulator during adipogenesis. Taken together, our work establishes HuR as an important posttranscriptional regulator of adipogenesis and provides insights into how RNA processing contributes to adipocyte development. Human antigen R (HuR) is an RNA binding protein that promotes mRNA stability. Here the authors show that HuR represses adipogenesis in white and brown adipose tissue by stabilizing Insig1 and other targets. |
| Databáze: | OpenAIRE |
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