A carbon nanotube-polymer composite for T-cell therapy
| Autor: | Tarek R, Fadel, Fiona A, Sharp, Nalini, Vudattu, Ragy, Ragheb, Justin, Garyu, Dongin, Kim, Enping, Hong, Nan, Li, Gary L, Haller, Lisa D, Pfefferle, Sune, Justesen, Kevan C, Herold, Kevin C, Harold, Tarek M, Fahmy |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Polymers
medicine.medical_treatment T cell T-Lymphocytes Cell Biomedical Engineering Cell Culture Techniques Cell- and Tissue-Based Therapy Bioengineering Nanotechnology Immunotherapy Adoptive Mice Cancer immunotherapy Antigen medicine Cytotoxic T cell Animals Humans General Materials Science Electrical and Electronic Engineering Antigens Melanoma Cells Cultured Cell Proliferation Cell growth Chemistry Nanotubes Carbon Growth factor Immunotherapy Condensed Matter Physics Atomic and Molecular Physics and Optics medicine.anatomical_structure Immobilized Proteins Biophysics Interleukin-2 |
| Zdroj: | Nature nanotechnology. 9(8) |
| ISSN: | 1748-3395 |
| Popis: | Clinical translation of cell therapies requires strategies that can manufacture cells efficiently and economically. One promising way to reproducibly expand T cells for cancer therapy is by attaching the stimuli for T cells onto artificial substrates with high surface area. Here, we show that a carbon nanotube-polymer composite can act as an artificial antigen-presenting cell to efficiently expand the number of T cells isolated from mice. We attach antigens onto bundled carbon nanotubes and combined this complex with polymer nanoparticles containing magnetite and the T-cell growth factor interleukin-2 (IL-2). The number of T cells obtained was comparable to clinical standards using a thousand-fold less soluble IL-2. T cells obtained from this expansion were able to delay tumour growth in a murine model for melanoma. Our results show that this composite is a useful platform for generating large numbers of cytotoxic T cells for cancer immunotherapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|