HIV-1 Nef protein causes death in stressed yeast cells due to determinants near the N-terminus and elsewhere in Nef

Ala change within Nef change caused increased toxicity. However, another determinant was also involved in toxicity since production of Nef20-206 was also still toxic. In each of these Nef-producing cells there was coincident membrane permeabilisation. These results suggest the possibility of a novel yeast bioassay for Nef inhibitors and that cells producing high levels of Nef may be selectively killed by stress. -->

ISSN:	1039-9712
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f7beeb6271a2606e45e60b4a00a80c8 https://pubmed.ncbi.nlm.nih.gov/9801796
Rights:	OPEN
Přírůstkové číslo:	edsair.doi.dedup.....8f7beeb6271a2606e45e60b4a00a80c8
Autor:	Cyril C. Curtain, Laura A. Castelli, Melinda G. Lowe, Ian Macreadie, Sonia E. Sankovich, Ahmed A. Azad, Paul Failla
Rok vydání:	1998
Předmět:	Programmed cell death Cell type Cell Membrane Permeability Copper Sulfate Hot Temperature viruses Clinical Biochemistry Saccharomyces cerevisiae Molecular Sequence Data Mutagenesis (molecular biology technique) Biochemistry Gene Products nef Transformation Genetic Genetics Amino Acid Sequence nef Gene Products Human Immunodeficiency Virus Molecular Biology Gene Peptide sequence biology virus diseases Cell Biology biology.organism_classification Virology Yeast Culture Media Genes nef Transformation (genetics) HIV-1 Mutagenesis Site-Directed
Zdroj:	ResearcherID
ISSN:	1039-9712
Popis:	The Human Immunodeficiency Virus type 1 (HIV-1) Nef protein is essential for AIDS pathogenesis. In order to determine more about the effects of Nef on basic cellular functions Nef was produced in yeast under a variety of conditions and in multiple cell types. Production of Nef caused cell death in acutely copper- or heat-stressed diploid cells. The N-terminal melittin-like region of Nef was involved in toxicity since a Trp5-->Ala change within Nef change caused increased toxicity. However, another determinant was also involved in toxicity since production of Nef20-206 was also still toxic. In each of these Nef-producing cells there was coincident membrane permeabilisation. These results suggest the possibility of a novel yeast bioassay for Nef inhibitors and that cells producing high levels of Nef may be selectively killed by stress.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f7beeb6271a2606e45e60b4a00a80c8 https://pubmed.ncbi.nlm.nih.gov/9801796 Zobrazit plný text záznamu Plný text