Features of Distamycin Preferential Binding Sites on Natural DNA Predicted Using Differential Scanning Calorimetry
| Autor: | Hiroyuki Ueta, Yoshio Kawai, Yoshimi Maeda |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Binding Sites
ColE1 Calorimetry Differential Scanning Stereochemistry Distamycins Organic Chemistry Bacteriocin Plasmids Distamycin DNA General Medicine Preferential binding Ligand (biochemistry) Applied Microbiology and Biotechnology Biochemistry DNA sequencing Analytical Chemistry chemistry.chemical_compound Crystallography Differential scanning calorimetry chemistry Binding site Molecular Biology Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 65:1261-1264 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.65.1261 |
| Popis: | The interaction of distamycin with ColE1 DNA was examined by using differential scanning calorimetry (DSC) taking the helix-coil transition theory of DNA into consideration. Our results here strongly indicate that the affinity of distamycin to DNA, at a low distamycin concentration, depends highly on the DNA sequence, and preferential binding occurs to the sites of four to six successive A-T pairs having two or more successive G-C pairs on both their ends. |
| Databáze: | OpenAIRE |
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