Bioluminescence-mediated longitudinal monitoring of adipose-derived stem cells in a large mammal ex vivo organ culture
| Autor: | Sjoerd van Rijn, Cornelis P. L. Paul, Marco N. Helder, David P. Noske, W. Peter Vandertop, Pieter-Paul A. Vergroesen, Thomas Wurdinger, Mirte Peeters |
|---|---|
| Přispěvatelé: | Neurosurgery, ANS - Amsterdam Neuroscience, Orthopedic Surgery and Sports Medicine, CCA - Disease profiling, MOVE Research Institute |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Survival Gene Expression Biology Organ culture Article Organ Culture Techniques In vivo Genes Reporter Transduction Genetic Cell Line Tumor medicine Bioluminescence imaging Animals Humans Luciferase Multidisciplinary Goats Stem Cells Mesenchymal stem cell Mesenchymal Stem Cells Cell biology Adipose Tissue Cell culture Cell Tracking Luminescent Measurements Female Stem cell Ex vivo |
| Zdroj: | Scientific Reports Peeters, M, van Rijn, S, Vergroesen, P P A, Paul, C P L, Noske, D P, Vandertop, W P, Wurdinger, T & Helder, M N 2015, ' Bioluminescence-mediated longitudinal monitoring of adipose-derived stem cells in a large mammal ex vivo organ culture ', Scientific Reports, vol. 5, 13960 . https://doi.org/10.1038/srep13960 Scientific reports, 5. Nature Publishing Group Scientific Reports, 5:13960. Nature Publishing Group |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep13960 |
| Popis: | Recently, ex vivo three-dimensional organ culture systems have emerged to study the physiology and pathophysiology of human organs. These systems also have potential as a translational tool in tissue engineering; however, this potential is limited by our ability to longitudinally monitor the fate and action of cells used in regenerative therapies. Therefore, we investigated luciferase-mediated bioluminescence imaging (BLI) as a non-invasive technique to continuously monitor cellular behavior in ex vivo whole organ culture. Goat adipose-derived stem cells (gADSCs) were transduced with either Firefly luciferase (Fluc) or Gaussia luciferase (Gluc) reporter genes and injected in isolated goat intervertebral discs (IVD). Luciferase activity was monitored by BLI for at least seven days of culture. Additionally, possible confounders specific to avascular organ culture were investigated. Gluc imaging proved to be more suitable compared to Fluc in monitoring gADSCs in goat IVDs. We conclude that BLI is a promising tool to monitor spatial and temporal cellular behavior in ex vivo organ culture. Hence, ex vivo organ culture systems allow pre-screening and pre-validation of novel therapeutic concepts prior to in vivo large animal experimentation. Thereby, organ culture systems can reduce animal use and improve the speed of innovation by overcoming technological, ethical and financial challenges. |
| Databáze: | OpenAIRE |
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