Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles
| Autor: | Vesna Vasić, Bojana Laban, Sandra Petrović, Uroš Ralević, D. Vasić-Anićijević, Mirjana Marković, Andreja Leskovac |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
inorganic chemicals
Silver education Metal Nanoparticles Nanoparticle 010402 general chemistry medicine.disease_cause 01 natural sciences Biochemistry Inorganic Chemistry Methionine Dynamic light scattering Microscopy medicine Zeta potential Humans Lymphocytes Fourier transform infrared spectroscopy L-methionine health care economics and organizations Cell Proliferation Cytokinesis Blood Cells Micronucleus Tests Toxicity 010405 organic chemistry Chemistry technology industry and agriculture Green Chemistry Technology respiratory system Catalase 0104 chemical sciences Oxidative Stress Colloidal gold Transmission electron microscopy Green Biophysics Nanoparticles Gold Genotoxicity |
| Zdroj: | Journal of Inorganic Biochemistry |
| Popis: | The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019 |
| Databáze: | OpenAIRE |
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