Establishing a mouse model of choroidal neovascularization to study the therapeutic effect of levotinib and its mechanism
| Autor: | Xiaonan Xin, Yueyu Zhu, Yuhua Hao, Ruijie Xi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Pathology medicine.medical_specialty genetic structures CNV 01 natural sciences Group A Article Retina 03 medical and health sciences chemistry.chemical_compound Levatinib medicine lcsh:QH301-705.5 business.industry Therapeutic effect Retinal VEGF eye diseases Staining Vascular endothelial growth factor Blot 030104 developmental biology Choroidal neovascularization medicine.anatomical_structure chemistry lcsh:Biology (General) sense organs medicine.symptom General Agricultural and Biological Sciences business Fluorescence leakage 010606 plant biology & botany |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 27, Iss 9, Pp 2491-2497 (2020) Saudi Journal of Biological Sciences |
| Popis: | Objective: To study the therapeutic effect and mechanism of levotinib on choroidal neovascularization (CNV) in mice. Methods: 45 healthy C57BL/6 mice were selected and randomly divided into three groups: control group (group A), model group (group B) and levotinib group (group C). The model of CNV in mice was established. The fluorescence leakage of choroidal lesions in mice was observed by fundus fluorescein angiography. The morphological changes of retinal vessels in mice were observed by retinal slice preparation, the pathological changes of eyeball tissues in mice were observed by hematoxylin-eosin (HE) staining, the expression of vascular endothelial growth factor (VEGF) in mice retina was detected by real-time quantitative fluorescence PCR, and the protein expression of VEGF in mice retina was detected by Western blotting. Result: On the 7th, 14th and 21st day after modeling, compared with group B, the fluorescence leakage area of group C mice was significantly reduced, and the difference was statistically significant (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|