Awa1p on the cell surface of sake yeast inhibits biofilm formation and the co-aggregation between sake yeasts and Lactobacillus plantarum ML11-11
| Autor: | Masashi Shimizu, Daisuke Watanabe, Soichi Furukawa, Hiroshi Takagi, Hitoshi Shimoi, Noriko Tsuchiya, Hirokazu Ogihara, Satoru Hirayama, Yasushi Morinaga |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Glycosylphosphatidylinositols Mutant Saccharomyces cerevisiae Bioengineering Applied Microbiology and Biotechnology Microbiology Cell wall Cell Wall Cell Adhesion URA3 Cell Aggregation Strain (chemistry) biology Alcoholic Beverages Biofilm Membrane Proteins biology.organism_classification Yeast Biochemistry Biofilms lipids (amino acids peptides and proteins) Lactobacillus plantarum Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 119:532-537 |
| ISSN: | 1389-1723 |
| DOI: | 10.1016/j.jbiosc.2014.10.007 |
| Popis: | We examined mixed-species biofilm formation between Lactobacillus plantarum ML11-11 and both foaming and non-foaming mutant strains of Saccharomyces cerevisiae sake yeasts. Wild-type strains showed significantly lower levels of biofilm formation compared with the non-foaming mutants. Awa1p, a protein involved in foam formation during sake brewing, is a glycosylphosphatidylinositol (GPI)-anchored protein and is associated with the cell wall of sake yeasts. The AWA1 gene of the non-foaming mutant strain Kyokai no. 701 (K701) has lost the C-terminal sequence that includes the GPI anchor signal. Mixed-species biofilm formation and co-aggregation of wild-type strain Kyokai no. 7 (K7) were significantly lower than K701 UT-1 (K701 ura3/ura3 trp1/trp1), while the levels of strain K701 UT-1 carrying the AWA1 on a plasmid were comparable to those of K7. The levels of biofilm formation and co-aggregation of the strain K701 UT-1 harboring AWA1 with a deleted GPI anchor signal were similar to those of K701 UT-1. These results clearly demonstrate that Awa1p present on the surface of sake yeast strain K7 inhibits adhesion between yeast cells and L. plantarum ML11-11, consequently impeding mixed-species biofilm formation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect