Characterization of the Escherichia coli protein-export gene secB
| Autor: | Anneliese K. Nault, Carol A. Kumamoto |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Molecular Sequence Data
DNA Recombinant Biology medicine.disease_cause Gene product Escherichia coli Genetics medicine Coding region Amino Acid Sequence Peptide sequence Gene Mutation Base Sequence Structural gene Nucleic acid sequence Chromosome Mapping General Medicine Precipitin Tests Molecular biology Open reading frame Genes Bacterial Electrophoresis Polyacrylamide Gel Bacterial Outer Membrane Proteins Plasmids |
| Zdroj: | Gene. 75:167-175 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(89)90393-4 |
| Popis: | The Escherichia coli secB gene product is required for normal export of envelope proteins out of the cell cytoplasm. In this report, we present the identification and nucleotide sequence of the secB coding sequence. The secB structural gene overlaps almost completely with a predicted open reading frame (ORF) that is encoded on the opposite strand. To establish the identity of the secB ORF, we characterized a secB mutation that caused total loss of secB function, based upon its phenotype. This mutation resulted from a nucleotide change that caused an ochre mutation in one ORF (the secB gene) and a silent (no amino acid change) codon change in the opposite ORF. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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