Immunohistochemical Staining for Proliferation Antigen as a Predictor of Chronic Graft Dysfunction and Renal Graft Loss
| Autor: | Michael M. Friedlaender, Galina Pizov |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Graft Rejection
Pathology medicine.medical_specialty medicine.drug_class Monoclonal antibody Antigen Predictive Value of Tests Humans Transplantation Homologous Medicine Kidney biology business.industry Graft Survival Antibodies Monoclonal Prognosis medicine.disease Immunohistochemistry Kidney Transplantation Transplantation Ki-67 Antigen medicine.anatomical_structure Monoclonal biology.protein Antibody business Cell Division Kidney disease |
| Zdroj: | Nephron. 92:738-742 |
| ISSN: | 2235-3186 1660-8151 |
| DOI: | 10.1159/000064104 |
| Popis: | Background: Assessment of proliferation rate (PR%) using monoclonal antibody for Ki-67 antigen has recently been found to have prognostic importance in lung and cardiac allografts. We ascertained whether the same might be true for renal allografts. Methods: Newly cut sections from 20 archival paraffin blocks of renal allograft biopsy material showing acute cellular rejection and/or acute tubular necrosis (ATN) and absence of other pathology were stained using MIB-1 antibody and were further double-stained with anti-CD3, anti-CD20 or anti-CD68 antibodies. Counts of staining of mononuclear interstitial cells were correlated with clinical and pathological data. Results: Mean PR% was significantly greater than that in control renal allografts (13.23 ± 1.94 vs. 2.84 ± 1.66, p < 0.01). PR% of cases with ATN and no or borderline rejection was significantly lower than that of the remaining cases with acute rejection pathology (6.68 ± 1.15 vs. 14.31 ± 1.62, p < 0.05). However, PR% was neither correlated to histological rejection grade nor to long-term graft outcome. Double labelling failed to identify the cell type of most infiltrating MIB-1 positive cells. Conclusion: Positive MIB-1 staining helps to identify the presence of rejection but does not appear to predict prognosis or correlate with the Banff classification of rejection pathology. |
| Databáze: | OpenAIRE |
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