Urinary metabolomic analysis of intrahepatic cholestasis of pregnancy based on high performance liquid chromatography/mass spectrometry
Autor: | Yong Shao, Xiaoqing Zhang, Min Ding, Li Ma, Ting Yang, Feng Pan, Yue Cui, Linlin Deng |
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Rok vydání: | 2017 |
Předmět: |
Adult
0301 basic medicine medicine.medical_specialty Metabolite Clinical Biochemistry Glycocholic acid Cholestasis Intrahepatic Biochemistry High-performance liquid chromatography Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolomics Pregnancy Internal medicine Chenodeoxycholic acid medicine Humans Hormone metabolism Human Metabolome Database Chromatography High Pressure Liquid 030219 obstetrics & reproductive medicine Chromatography Biochemistry (medical) General Medicine medicine.disease Pregnancy Complications 030104 developmental biology Endocrinology chemistry Case-Control Studies Multivariate Analysis Metabolome Female Cholestasis of pregnancy |
Zdroj: | Clinica Chimica Acta. 471:292-297 |
ISSN: | 0009-8981 |
Popis: | Intrahepatic cholestasis of pregnancy (ICP), a pregnancy-related liver disease, leads to complications for both mothers and fetuses. Metabolomic approach has been applied to maternal-fetal medicine. The global metabolomic alterations that are specific in ICP as yet have not been investigated.Based on high performance liquid chromatography/hybrid quadrupole time-of-flight (HPLC/Q-TOF) mass spectrometry, the untargeted metabolomics was used to analyze the changes of urinary metabolites between ICP group and the control group.One hundred nine variables in positive model and 119 variables in negative model were significantly different (p0.05) between the ICP group and the control group, with the VIP (variable importance in the project) score1 by the orthogonal partial least squares discriminant analysis (OPLS-DA). 14 metabolites in positive model and 18 metabolites in negative model were selected and identified based on HMDB (human metabolome database). Most of these metabolites were involved in bile acids biosynthesis and metabolism, hormone metabolism and lipid metabolism. A metabolite panel (MG (22:5), LysoPE (22:5), l-homocysteine sulfonic acid, glycocholic acid and chenodeoxycholic acid 3-sulfate) was contrusted by the binary logistic regression analysis with high diagnostic accuracy for ICP. The area under the receiver operating characteristic curve was 0.988 with the sensitivity of 90.0% and specificity of 93.3%.Urinary metabolites allow for the discrimination of ICP from the controls by orthogonal partial least squares discriminant analysis. Therefore, these findings may provide deep insights for the etiopathogenesis of ICP. Moreover, the maternal urinary metabolite panel has the potential to be used as non-invasive biomarkers for the diagnosis of ICP. |
Databáze: | OpenAIRE |
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