Activating mutations in c-KIT and PDGFRα are exclusively found in gastrointestinal stromal tumors and not in other tumors overexpressing these imatinib mesylate target genes
| Autor: | Hans P de Schipper, Harry F G M van den Ingh, Erik A.C. Wiemer, Erwin van der Harst, Hans van Tol, Alex M de Bruin, Wolter Oosterhuis, Kees Nooter, Johan M. Kros, Herman Burger, Michael A. den Bakker |
|---|---|
| Přispěvatelé: | Medical Oncology, Pathology, Surgery |
| Rok vydání: | 2005 |
| Předmět: |
Cancer Research
Receptor Platelet-Derived Growth Factor alpha Gastrointestinal Stromal Tumors medicine.drug_class DNA Mutational Analysis Antineoplastic Agents medicine.disease_cause Piperazines Tyrosine-kinase inhibitor Receptor tyrosine kinase Exon medicine Humans neoplasms Pharmacology Mutation biology GiST Imatinib Enzyme Activation Proto-Oncogene Proteins c-kit Pyrimidines Imatinib mesylate Oncology Benzamides Imatinib Mesylate biology.protein Cancer research Molecular Medicine Platelet-derived growth factor receptor medicine.drug |
| Zdroj: | Scopus-Elsevier Cancer Biology & Therapy, 4(11), 1270-1274. Landes Bioscience |
| ISSN: | 1555-8576 1538-4047 |
| DOI: | 10.4161/cbt.4.11.2253 |
| Popis: | Previous studies have shown that Imatinib mesylate (Gleevec), a selective tyrosine kinase inhibitor of c-KIT and platelet-derived growth factor receptors (PDGFR), is highly effective in c-KIT/CD117-positive gastrointestinal stromal tumors (GIST), especially in those having activating mutations in c-kit exon 11. In addition, gain-of-function mutations in the juxtamembrane domain (exon 12) and the kinase activation loop (exon 18) of PDGFRalpha were found in GISTs. Importantly, the presence and type of these mutually exclusive c-KIT or PDGFRalpha mutations were found to be associated with the response to imatinib. Here, we examined the prevalence of c-kit exon 11 and PDGFRalpha exons 12 and 18 mutations in other tumor types known to express these tyrosine kinase receptors in order to explore which other cancer types may potentially benefit from imatinib treatment. We determined the mutational status of these commonly mutated exons by direct sequencing in 11 different tumor types (in total: 215 unrelated cases), including GIST, chordoma, and various distinct tumors of lung, brain and its coverings, and skin cancer. Of the 579 exons examined (211 c-kit exon 11, 192 PDGFRalpha exon 12, 142 PDGFRalpha exon18, 17 PDGFRbeta exon 12 and 17 PDGFRbeta exon 18), only 12 (all GIST) harbored mutations (10 c-kit exon 11 and 2 PDGFRalpha exon18). From these data we conclude that activating c-KIT and PDGFR mutations are sporadic in human cancers known to overexpress these tyrosine kinase receptor genes and suggest that, except in GIST, this overexpression is not correlated with activating mutations. The latter may imply that these wild-type c-KIT and PDGFR tumor types will probably not benefit from imatinib treatment. |
| Databáze: | OpenAIRE |
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