Expression of PEP-19 inhibits apoptosis in PC12 cells
| Autor: | Legos Jj, Wang X, Erhardt Ja, Slemmon, Johanson Ra |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Programmed cell death
Ultraviolet Rays education Apoptosis Nerve Tissue Proteins Biology PC12 Cells medicine Staurosporine Animals Protein kinase A Neurons L-Lactate Dehydrogenase General Neuroscience Depolarization Molecular biology Chromatin Rats Enzyme Activation Cell culture Calcium-Calmodulin-Dependent Protein Kinases cardiovascular system Calmodulin-Binding Proteins Signal transduction Calcium-Calmodulin-Dependent Protein Kinase Type 2 circulatory and respiratory physiology medicine.drug |
| Zdroj: | Neuroreport. 11(17) |
| ISSN: | 0959-4965 |
| Popis: | PEP-19 is a calmodulin-regulatory protein found specifically within neurons, though cellular functions of this protein have not been determined. In an effort to define potential effects of PEP-19, PC12 cell lines expressing this protein were generated and subjected to apoptotic stimuli. As measured by LDH release, cell death in PEP-19 expressing cells was 2- to 5-fold less following u.v. irradiation, and 2- to 4-fold less following staurosporine treatment than controls. Additionally, PEP-19-expressing cells displayed decreased DNA ladder formation, chromatin and condensation, caspase activation following staurosporine treatment. Overall, these results demonstrate that PEP-19 can inhibit apoptotic processes in PC12 cells, suggesting a potential regulatory mechanism for pathways leading to cell death. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|