Susceptibility of zebra fish Danio rerio to infection by Flavobacterium columnare and F. johnsoniae
| Autor: | Thomas R. Moyer, David W. Hunnicutt |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Bacterial gliding
Lethal dose Danio Aquatic Science Biology biology.organism_classification Median lethal dose Flavobacteriaceae Flavobacterium Survival Analysis Microbiology Lethal Dose 50 Fish Diseases Flavobacteriaceae Infections RNA Ribosomal 16S Flavobacterium columnare Animals Disease Susceptibility Pathogen Ecology Evolution Behavior and Systematics Bacteria Zebrafish |
| Zdroj: | Diseases of aquatic organisms. 76(1) |
| ISSN: | 0177-5103 |
| Popis: | Flavobacterium columnare is a serious pathogen in a wide range of fish species. F. johnsoniae is an opportunistic pathogen of certain fish. Both are gliding bacteria. These species were tested for their ability to infect the zebra fish Danio rerio. Both injection and bath infection methods were tested. The results indicate that F. johnsoniae is not an effective pathogen in D. rerio, but that F. columnare is an effective pathogen. F. johnsoniae did not cause increased death rates following bath infection, but did cause increased death rates following injection, with an LD 50 (mean lethal dose) of approximately 3 x 10 10 cfu (colony-forming units). Non-motile mutants of F. johnsoniae produced a similar LD 50 . F. columnare caused increased death rates following both injection and bath infections. There was considerable strain variation in LD 50 , with the most lethal strain tested producing an LD 50 of 3.2 x 10 6 cfu injected and 1.1 x 10 6 cfu ml -1 in bath experiments, including skin damage. The LD 50 of F. columnare in zebra fish without skin damage was >1 x 10 8 , indicating an important effect of skin damage. |
| Databáze: | OpenAIRE |
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