High Prevalence of ST131 Among CTX-M-ProducingEscherichia colifrom Community-Acquired Infections, in the Czech Republic

Autor: Jaroslav Hrabak, Vladislav Jakubů, Costas C. Papagiannitsis, Vendula Studentova, Helena Žemličková, Petra Španělová, Pavla Urbášková
Rok vydání: 2015
Předmět:
Male
medicine.medical_treatment
Drug resistance
medicine.disease_cause
Plasmid
Disk Diffusion Antimicrobial Tests
Drug Resistance
Multiple
Bacterial
Prevalence
polycyclic compounds
Child
Escherichia coli Infections
Phylogeny
Czech Republic
Aged
80 and over
Genetics
Virulence
Escherichia coli Proteins
Middle Aged
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
Community-Acquired Infections
Ciprofloxacin
Child
Preschool
Conjugation
Genetic
Female
Fluoroquinolones
Plasmids
medicine.drug
Adult
DNA
Bacterial
Microbiology (medical)
Adolescent
Sequence analysis
Immunology
Biology
Microbiology
beta-Lactamases
Escherichia coli
medicine
Humans
Genotyping
Aged
Pharmacology
Infant
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Clone Cells
Genes
Bacterial
Beta-lactamase
Multilocus sequence typing
Multilocus Sequence Typing
Zdroj: Microbial Drug Resistance. 21:74-84
ISSN: 1931-8448
1076-6294
Popis: A total of 2,683 nonrepetitive Escherichia coli isolates were collected from microbiological laboratories covering all regions of the Czech Republic, during April 2011. Antimicrobial susceptibility patterns of E. coli were assessed. All 38 cefotaxime-resistant (CTX-R) isolates were found to be extended-spectrum β-lactamase (ESBL)-positive by the double-disc synergy test. Thirty-two of those isolates produced enzymes of CTX-M-1 family, five of CTX-M-9 family, and one isolate both CTX-M types. Genotyping by multilocus sequence typing classified all ESBL-producing isolates into 13 sequence types (STs). ST131 was the most prevalent and was exclusively correlated with E. coli belonging to the more-virulent phylogroup B2. blaCTX-M-15 and blaCTX-M-9-like genes were mainly carried by plasmids belonging to the IncF group, while replicon I1 was predominant among CTX-M-1-encoding plasmids. Additionally, 63% of the ESBL-producing isolates were also resistant to ciprofloxacin. Sequence analysis of quinolone resistance-determining regions of gyrA and parC revealed the presence of amino acid substitutions in 22 out of 23 ciprofloxacin-resistant isolates. The acc(6')-Ib-cr and qnrB1 plasmid-mediated quinolone resistance genes were also detected in some of the isolates. This is the first report on the emergence and spread of CTX-M-producing E. coli in the community of the Czech Republic, indicating the high prevalence of ST131 clone among CTX-M producers.
Databáze: OpenAIRE
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