Functional evaluation of the grafted wall with porcine-derived small intestinal submucosa (SIS) to a stomach defect in rats
| Autor: | Kenichiro Uemura, Marcia R. Gottfried, Sebastian G. de la Fuente, Christopher R. Mantyh, Tomio Ueno, Mary B. Harris, Toku Takahashi, Theodore N. Pappas, Makoto Tatewaki, Omar Abdel-Wahab, D. Curtis Lawson |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Nitroprusside Pathology medicine.medical_specialty Carbachol Swine Muscle Relaxation Transplantation Heterologous Stimulation Cholinergic Agonists Cholinergic Antagonists Rats Sprague-Dawley Stomach surgery Cell Movement Submucosa Intestine Small Animals Medicine Intestinal Mucosa business.industry Stomach Muscle Smooth Anatomy Electric Stimulation Small intestine Rats Transplantation medicine.anatomical_structure Models Animal Tissue Transplantation Surgery Sodium nitroprusside business Muscle Contraction medicine.drug |
| Zdroj: | Surgery. 142:376-383 |
| ISSN: | 0039-6060 |
| DOI: | 10.1016/j.surg.2007.04.019 |
| Popis: | Small intestinal submucosa (SIS) represents a novel bio-scaffolding material that may be used to repair hollow-organ defects. However, it is unclear whether neurophysiologic responses return to SIS-grafted areas in the gut. We evaluated the functional recovery of a stomach defect grafted with the porcine-derived SIS.Twelve rats had a full-thickness defect created in the stomach. SIS was secured to the gastric wall. After 6 months, muscle strips were harvested from within the grafted area to perform both a histologic and a functional study. Additional full-thickness muscle strips were harvested from the posterior in the same stomach as controls. A dose response curve was obtained with carbachol (CCH) or sodium nitroprusside (SNP). Activation of intrinsic nerves was achieved by electrical field stimulation (EFS).The response to CCH and amplitude in EFS showed tonic contraction in both controls and SIS strips in a concentration-dependent and frequency-dependent manner. The magnitude after each stimulation was significantly lower in SIS strips compared with controls (P.01). However, the contraction ratio of EFS to ED(50) of CCH was not significantly different between the groups. Additionally, SNP produced relaxation in both strips in a concentration-dependent manner. Histologic findings revealed that an insufficient amount of smooth-muscle cells existed in the muscularis propria, whereas compensated growth was observed in the submucosa with nerve regeneration.This study demonstrates that SIS provides a template for nerve migration to the graft in the rodent stomach. Innervations showed a similar distribution to that observed in the controls. The clinical implications of such findings warrant additional investigation. |
| Databáze: | OpenAIRE |
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