Elucidation of LSD In Vitro Metabolism by Liquid Chromatography and Capillary Electrophoresis Coupled with Tandem Mass Spectrometry*
| Autor: | Jianyi Cai, Jack D. Henion |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Health
Toxicology and Mutagenesis Metabolite Urine In Vitro Techniques Toxicology Tandem mass spectrometry Gas Chromatography-Mass Spectrometry Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Humans Environmental Chemistry Chemical Health and Safety Chromatography Chemistry Electrophoresis Capillary Metabolism Reference Standards Lysergic acid Lysergic Acid Diethylamide Biochemistry Microsomes Liver Microsome Drug metabolism Chromatography Liquid |
| Zdroj: | Journal of Analytical Toxicology. 20:27-37 |
| ISSN: | 1945-2403 0146-4760 |
| Popis: | The in vitro metabolism of D-lysergic acid diethylamide (LSD) by human liver microsomes was investigated. Tandem mass spectrometric techniques, using precursor and neutral loss scans, were employed in the initial search for drug metabolites. The determination of LSD human liver in vitro metabolites was performed by high-performance liquid chromatography and capillary electrophoresis coupled with tandem mass spectrometry. Two new in vitro metabolites, lysergic acid ethylamide (LAE) and 2-oxo-LSD, were positively identified; their structures were established by comparing with reference standards. Several other possible in vitro metabolites detected were suggested to be mono- and trioxylated metabolites of LSD. The major metabolic route of LSD by human liver microsomes is deethylation. Some results with LSD-positive human urine are presented. Among the LSD-related compounds detected in human urine specimens, iso-LSD was present at the highest concentration, followed by nor-LSD and isonor-LSD. Low concentrations of LAE and iso-LAE were also found in these urine specimens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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