A Graded, Porous Composite of Natural Biopolymers and Octacalcium Phosphate Guides Osteochondral Differentiation of Stem Cells

Autor: Elisabeth Amann, Elizabeth R. Balmayor, Isabel B. Leonor, Gastón Fuentes Estévez, Martijn van Griensven, Amisel Amirall, Rui L. Reis, Francisco J. Sola Dueñas, Patrina S. P. Poh, Albina R. Franco
Přispěvatelé: CBITE, RS: MERLN - Cell Biology - Inspired Tissue Engineering (CBITE), RS: MERLN - Instructive Biomaterials Engineering (IBE), Division Instructive Biomaterials Eng, Universidade do Minho
Rok vydání: 2021
Předmět:
Calcium Phosphates
bones
Scaffold
CROSS-LINKING
Biomedical Engineering
Pharmaceutical Science
02 engineering and technology
010402 general chemistry
bone
SCAFFOLDS
01 natural sciences
Chondrocyte
Biomaterials
Extracellular matrix
chemistry.chemical_compound
medicine
Humans
multiphasic scaffolds
cartilage
Octacalcium phosphate
CHITOSAN
REPAIR
Science & Technology
Tissue Engineering
Tissue Scaffolds
TRANSPLANTATION
Chemistry
Stem Cells
cartillage
Cartilage
Mesenchymal stem cell
chitosan-collagen-octacalcium phosphate composites
Cell Differentiation
osteochondral differentiation
ARTICULAR-CARTILAGE
021001 nanoscience & nanotechnology
HYDROXYAPATITE
ddc
0104 chemical sciences
Transplantation
medicine.anatomical_structure
osteochondral differenctiation
OSTEOARTHRITIS
Biophysics
CHONDROCYTE
Stem cell
BONE
0210 nano-technology
Porosity
Zdroj: Advanced Healthcare Materials, 10(6):2001692. Wiley
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
ISSN: 2192-2659
2192-2640
DOI: 10.1002/adhm.202001692
Popis: Lesions involving the osteochondral unit are difficult to treat. Biomimetic scaffolds are previously shown as promising alternative. Such devices often lack multiple functional layers that mimic bone, cartilage, and the interface. In this study, multilayered scaffolds are developed based on the use of natural extracellular matrix (ECM)â like biopolymers. Particular attention is paid to obtain a complex matrix that mimics the native osteochondral transition. Porous, spongeâ like chitosanâ collagenâ octacalcium phosphate (OCP) scaffolds are obtained. Collagen content increases while the amount of OCP particles decreases toward the cartilage layer. The scaffolds are bioactive as a mineral layer is deposited containing hydroxyapatite at the bony side. The scaffolds stimulate proliferation of human adiposeâ derived mesenchymal stem cells, but the degree of proliferation depends on the cell seeding density. The scaffolds give rise to a zoneâ specific gene expression. RUNX2, COL1A1, BGLAP, and SPP1 are upregulated in the bony layer of the scaffold. SOX9 is upregulated concomitant with COL2A1 expression in the cartilage zone. Mineralization in presence of the cells is prominent in the bone area with Ca and P steadily increasing over time. These results are encouraging for the fabrication of biomimetic scaffolds using ECMâ like materials and featuring gradients that mimic native tissues and their interface.
E.A. thanks the TUM Graduate School for funding her research stay at the 3B’s Research Group, University of Minho (Guimarães, Portugal). This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the project “ChimericFibre4Tendon” PTDC/BIIBIO/28870/2017.
Databáze: OpenAIRE
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