Cloning of Dengue Virus Type 2 by the Direct Fluorescent Antibody Technique
| Autor: | A. S. Lubiniecki, William McD. Hammon, Tarr Gc |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Cloning
Virus Cultivation medicine.diagnostic_test Fluorescent Antibody Technique Dengue Virus Dengue virus Biology Kidney medicine.disease_cause Immunofluorescence medicine.disease Virology General Biochemistry Genetics and Molecular Biology Cell Line Dengue fever Immune system Cricetinae medicine Animals Clone (B-cell biology) Antigens Viral Direct fluorescent antibody |
| Zdroj: | Experimental Biology and Medicine. 144:70-72 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-144-37529 |
| Popis: | SummaryDengue virus type 2 has been cloned in hamster kidney cells, a noncyto-pathic, noncytocidal virus-cell system, utilizing a direct fluorescent antibody method. A clone is considered to have arisen from the infection of a susceptible cell by a single infectious particle of DEN-2.This work was performed under the sponsorship of the Commission on Viral Infections, Armed Forces Epidemiological Board, and was supported by the U. S. Army Medical Research and Development Command under Contract No. DADA17-69-C-9048 and by Public Health Service Research Grant No. AI-02686 from the National Institute of Allergy and Infectious Diseases. The application of direct fluorescent antibody methods to dengue viruses was achieved by Drs. Robert W. Atchison and J. Jayavasu, who also prepared the conjugated immune ascitic fluids. Most able technical assistance was provided by Alma Tillman, Annie McCoy, Carlie White, and Frances Brisset. |
| Databáze: | OpenAIRE |
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